The acetylcholine receptor γ-to-ε switch occurs in individual endplates
The acetylcholine receptor γ-to-ε switch occurs in individual endplates
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DOI：
10.1016/j.bbrc.2005.04.081
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发表时间：
2005-06
影响因子：
3.1
通讯作者：
Norihiro Yumoto;S. Wakatsuki;A. Sehara-Fujisawa
中科院分区：
文献类型：
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作者：
Norihiro Yumoto;S. Wakatsuki;A. Sehara-Fujisawa
神经肌肉接头的成熟伴随着乙酰胆碱受体（AChR）通道的分子转换，从胚胎型的γ-亚基到出生后的成人型的ε-亚基。作为理解γ-到-ε转换的分子机制的一步，我们解决了胚胎型和成人型AChR在过渡期是否构成不同终板的问题。通过用抗-γ-和/或抗-ε-抗体以及与α-亚基结合的α-银环蛇毒素进行双重或三重染色的分析，我们证明了在小鼠的新生期，成人型AChR被掺入表达胚胎AChR的单个终板中，并逐渐取代这些胚胎AChR。AChR在小鼠膈肌过渡的主要时期是出生后第5天（P5）和P7之间，类似于先前描述的期间，其中终板从多轴突神经支配转变为单轴突神经支配。这一发现将有助于我们理解神经肌肉接头建立过程中γ-到-ε转换的机制。
Maturation of the neuromuscular junction is accompanied by molecular switching of acetylcholine receptor (AChR) channels from embryonic types with γ-subunits to adult ones with ε-subunits after birth. As a step toward understanding the molecular mechanisms of the γ-to-ε switch, we addressed the question of whether embryonic- and adult-type AChRs constitute different endplates during the transitional period. From analyses with double- or triple-staining with anti-γ- and/or anti-ε-antibodies together with α-bungarotoxin, which binds to α-subunits, we demonstrated that during neonatal stages in mice, adult-type AChRs are incorporated into individual endplates expressing embryonic-AChRs and replace these embryonic-AChRs gradually. The main period of AChR transition in the mouse diaphragm was between postnatal days 5 (P5) and P7, similar to the period described previously in which endplates shift from multi-axon to single-axon innervation. This finding will help our understanding of the mechanisms of the γ-to-ε switch during establishment of the neuromuscular junction.