Tenascin-C concentration in synovial fluid correlates with radiographic progression of knee osteoarthritis.
Tenascin-C concentration in synovial fluid correlates with radiographic progression of knee osteoarthritis.
复制标题
滑液中腱蛋白-C 的浓度与膝骨关节炎的放射学进展相关。
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
A. Uchida
中科院分区：
文献类型：
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作者：
M. Hasegawa;H. Hirata;A. Sudo;K. Kato;Daisuke Kawase;N. Kinoshita;Toshimichi Yoshida;A. Uchida
客观化
骨关节炎(OA)是导致残疾的主要原因，也是老龄化人口中最常见的疾病。虽然通常使用标准的放射图像来评估疾病的病程，但也可以使用生化标记物来检测疾病并确定其严重程度。我们建立了一种针对TN-C大剪接变异体(TN-C)的单抗的酶联免疫吸附试验(EL ISA)系统，并检测了滑液(SF)中的TN-C是否是反映OA进展的合适的生化标志物。
方法
SF样本取自74例骨关节炎患者和16例非骨关节炎患者的膝关节。根据膝关节骨性关节炎严重程度的X线分级，将膝关节分为轻、中、重度三组。免疫印迹法检测TN-C剪接变异体的表达。用双抗体夹心法测定TN-C浓度。
结果
Western blotting显示严重骨性关节炎的SF中存在较大的TN-C变异体。与未发生骨性关节炎的受试者相比，骨性关节炎患者的TN-C水平高出5倍(p<；0.0001)。TN-C水平在对照组与轻度骨性关节炎之间无明显差异，但在中度(p=0.0244)和重度(p<；0.0001)骨性关节炎患者中显著升高。在根据年龄、体重指数和性别调整TN-C水平后，TN-C水平与膝关节骨关节炎的X线进展相关(R2=0.404，p<；0.0001)。
结论
TN-C，包括大的变异亚基，是疾病后期OA进展的有用的生化标记物。
OBJECTIVE
Osteoarthritis (OA) is a major cause of disability and represents the most common disease in the aging population. Although the course of the disease is generally assessed using standard radiographic images, biochemical markers may be employed to detect the disease and determine the degree of severity. We developed an enzyme linked immunosorbent assay (ELISA) system using a monoclonal antibody specific for the large-splice variants of tenascin-C (TN-C) and examined whether TN-C in synovial fluid (SF) is an adequate biochemical marker of OA progression.
METHODS
SF samples were obtained from knees of 74 patients with OA and 16 without OA. Based on the radiographic grading of the OA severity, the knees were divided into 3 groups: mild, moderate, and severe OA. Expression of TN-C splice variants was examined using immunoblotting. TN-C concentrations were determined by ELISA.
RESULTS
Western blotting showed the presence of large TN-C variants in SF from severe OA. TN-C levels were 5-fold higher in OA samples compared to subjects without OA (p < 0.0001). TN-C levels were not different between control cases and mild OA, but increased significantly in moderate (p = 0.0244) and severe OA (p < 0.0001). After adjusting TN-C levels for age, body mass index, and sex, TN-C levels correlated with radiographic progression of knee OA (R2 = 0.404, p < 0.0001).
CONCLUSION
TN-C, including the large-variant subunits, is a useful biochemical marker for OA progression in the later stages of disease.