Course of murine leukemia retrovirus infection determined in vivo by magnetic resonance imaging.
Course of murine leukemia retrovirus infection determined in vivo by magnetic resonance imaging.
复制标题
通过磁共振成像确定体内小鼠白血病逆转录病毒感染的过程。
作者：
Allegrini Pr;Wachsmuth Ed
应用磁共振成像技术在体内测量三种不同白血病逆转录病毒（LP-BM5小鼠白血病病毒、Friend和Rauscher）感染小鼠脾脏和淋巴结的体积。快速腹腔注射番红花麻醉对磁共振成像是足够的，并可在适当的间隔重复。同时获取11张经腹部的额叶磁共振图像，相邻切片中心距离为1.5 mm。为了更好地区分脾脏与周围组织，对感染LP-BM5小鼠白血病病毒的小鼠进行轻度t2加权磁共振成像，对感染Friend和Rauscher病毒的小鼠进行轻度t1加权磁共振成像。在每组24 - 28只小鼠中，测量只需要3 - 4小时，通过使用标准化支架(i)容纳两只动物平卧位，（ii）确保在磁共振仪器中可重复定位，以及（iii）通过将轻度t2加权磁共振图像的相位编码步骤从256减少到128。脾脏和腹股沟淋巴结的体积由各自的横截面积计算。脾脏和腹股沟淋巴结的重量与磁共振成像体积相关性较好（r > 0.95）。尽管脾肿大和淋巴结病变的程度在任何给定时间都有很大的变化，但疾病的进展可以很容易地通过间隔重复磁共振成像来跟踪。因此，在只需要使用少数动物的感染模型中就可以获得统计上相关的结果。
Magnetic resonance imaging was applied to measure the volumes of spleens and lymph nodes of mice infected with three different leukemia retroviruses (LP-BM5 murine leukemia virus, Friend, and Rauscher) in vivo. Anesthesia by rapid intraperitoneal injection of Saffan was sufficient for magnetic resonance imaging and could be repeated at appropriate intervals. Eleven frontal magnetic resonance images through the abdomen with a center-to-center distance of 1.5 mm between adjacent slices were acquired simultaneously. To optimally demarcate spleens from the surrounding tissues, the magnetic resonance images were mildly T2-weighted for mice infected with LP-BM5 murine leukemia virus and mildly T1-weighted for those infected with Friend and Rauscher virus. Measurements requiring only 3 to 4 hours in groups of 24 to 28 mice were accomplished by using a standardized holder (i) accommodating two animals in the supine position and (ii) ensuring reproducible positioning in the magnetic resonance-instrument, and (iii) by reducing the number of phase-encoding steps of mildly T2-weighted magnetic resonance images from 256 to 128. Volumes of spleens and inguinal lymph nodes were calculated from the respective cross-sectional areas. The weights and magnetic resonance image-derived volumes of spleens and inguinal lymph nodes correlated well (r greater than 0.95). Despite large variations in the extent of splenomegaly and lymphadenopathy at any given time, the progression of the disease could easily be followed by repeating magnetic resonance imaging at intervals. Thus, statistically relevant results can be obtained in an infection model requiring the use of only a few animals.