Utilization of shrimp-shell waste as a substrate for the activity of chitinases produced by microorganisms.
Utilization of shrimp-shell waste as a substrate for the activity of chitinases produced by microorganisms.
复制标题
利用虾壳废物作为微生物产生的几丁质酶活性的底物。
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
W. Donderski
中科院分区：
文献类型：
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作者：
M. Brzezinska;M. Walczak;E. Lalke;W. Donderski
几丁质分解微生物，如细菌，真菌和放线菌，分离自水和底沉积物的Chelmzynskie湖及其流域的土壤。研究了不同形式的几丁质对这些微生物降解几丁质活性的影响。从分析的环境中分离的细菌表现出最高的几丁质分解活性的存在下，胶体几丁质。在含有虾壳的培养基中也观察到高活性。放线菌几丁质酶活性分析表明，从土壤中分离的放线菌比从湖水和底泥中分离的放线菌具有更高的几丁质酶活性。所有土壤放线菌在含有胶体甲壳素和虾壳的培养基中表现出最高的活性。在水和底部沉积物中收集的放线菌的特点是相似的活动水平。几丁质分解真菌也表现出最大的活动在介质中含有胶体几丁质。培养基中虾壳的存在对几丁质分解活性的影响最小。在所分析的甲壳素形式中，甲壳素粉末对甲壳素酶活性的影响最小。
Chitinolytic microorganisms such as bacteria, fungi, and actinomycetes, were isolated from the water and bottom sediments of Lake Chelmzynskie and the soil of its watershed. The impact of various forms of chitin on the chitinolytic activity of these microorganisms was investigated. Bacteria isolated from the analyzed environments exhibited the highest chitinolytic activity in the presence of colloidal chitin. High activity was also observed in a culture medium containing shrimp shells. The analysis of chitinolytic activity of actinomycetes demonstrated that strains isolated from the soil had considerably higher activity levels than those isolated from lacustrine water and bottom sediments. All soil actinomycetes exhibited the highest activity in a culture medium containing colloidal chitin and shrimp shells. Actinomycetes collected in water and bottom sediments were characterized by similar activity levels. Chitinolytic fungi exhibited the maximum activity also in a medium containing colloidal chitin. The presence of shrimp shells in a culture medium had the lowest impact on chitinolytic activity. Among the analyzed forms of chitin, the impact of chitin powder on the activity of chitinases was the least stimulating.