Microbial-mediated release of bisphenol A from polycarbonate vessels
Microbial-mediated release of bisphenol A from polycarbonate vessels
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DOI：
10.1111/j.1472-765x.2007.02301.x
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发表时间：
2008-02-01
影响因子：
2.4
通讯作者：
Falkinham, J. O., III
中科院分区：
文献类型：
--
作者：
Oberlies, N. H.;Li, C.;Falkinham, J. O., III
目的：确定产抗生素芽孢杆菌培养物中双酚 A (BPA) [2,2'-双（4-羟基苯基）丙烷] 的来源。菌株在聚碳酸酯烧瓶中生长。方法和结果：虽然产生抗生素的芽孢杆菌属的培养物。在新的、经过冲洗的聚碳酸酯烧瓶中生长的菌株产生 BPA，而在彻底清洗过的聚碳酸酯烧瓶中生长的重复培养物则不会。大肠杆菌 C 株细胞在新的聚碳酸酯烧瓶中生长，用 100 ml 蒸馏水冲洗 3 次。 BPA 只能从新聚碳酸酯烧瓶中生长的培养物中回收，而不能从平行培养的高压灭菌培养基中回收。结论：BPA 存在于芽孢杆菌或大肠杆菌培养物中，可能是由于其从未充分清洗的聚碳酸酯烧瓶中释放出来。标准高压灭菌不会导致 BPA 出现；需要微生物的生长。用于微生物培养的聚碳酸酯容器应彻底清洗，以避免培养基中出现 BPA。 研究的意义和影响：本研究严格证明，培养基中 BPA 的存在是未充分清洗的聚碳酸酯烧瓶中微生物生长或代谢的结果。由于 BPA 具有抗菌和雌激素活性，因此新药的搜索或重组化疗药物的生产可能会因 BPA 的人工出现而脱轨。
Aim: To identify the source of bisphenol A (BPA) [2,2'-bis(4-hydroxyphenyl) propane] in cultures of an antibiotic-producing Bacillus sp. strain grown in polycarbonate flasks.Methods and Results: Although a culture of an antibiotic-producing Bacillus sp. strain grown in a new, rinsed polycarbonate flask yielded BPA, duplicate cultures grown in thoroughly washed polycarbonate flasks did not. Cells of Escherichia coli strain C were grown in new polycarbonate flasks rinsed three-times with 100 ml distilled H2O. BPA was only recovered from cultures grown in new polycarbonate flasks, but not from the autoclaved medium incubated in parallel.Conclusions: BPA was present in either Bacillus or E. coli cultures, probably due to its release from inadequately washed polycarbonate flasks. Standard autoclaving did not result in BPA appearance; microbial growth was required. Polycarbonate vessels for microbial cultures should be thoroughly washed to avoid the appearance of BPA in culture medium.Significance and Impact of the Study: This study rigorously demonstrates that the presence of BPA in culture medium was a consequence of microbial growth or metabolism in inadequately washed polycarbonate flasks. As BPA exhibits antimicrobial and oestrogenic activity, searches for novel drugs or production of recombinant chemotherapeutic agents could be derailed by the artefactual appearance of BPA.