The cellobiose and α-ketoglutarate as metabolic modulators in Eriocheir sinensis to resist Spiroplasma eriocheiris infection
The cellobiose and α-ketoglutarate as metabolic modulators in Eriocheir sinensis to resist Spiroplasma eriocheiris infection
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纤维二糖和α-酮戊二酸作为中华绒螯蟹代谢调节剂抵抗绒毛虫感染
DOI：
10.1016/j.aquaculture.2020.736180
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发表时间：
2021-02-25
期刊：
影响因子：
4.5
通讯作者：
Meng, Qingguo
中科院分区：
文献类型：
--
作者：
Cao, Cheng;Liu, Zhanghuai;Meng, Qingguo
由绒毛虫引起的震颤病是中华绒螯蟹危害最大的疾病之一，极大地增加了养殖业的经济损失。随着生物技术的发展，代谢组学在筛选与生理和病理变化相关的代谢物方面发挥着重要作用。本研究采用基于GC/MS的代谢组学方法，对中华绒螯蟹不同表型组(震颤组、无症状组和对照组)的血细胞代谢产物进行了研究。与震颤组比较，无症状组42种差异代谢物(26种上调，16种下调)差异有统计学意义。KEGG途径富集法分析表明，差异代谢物主要集中在“ABC转运蛋白”、“维生素B6代谢”、“抗坏血酸和杏仁酸代谢”和“淀粉和蔗糖代谢”中。选择纤维二糖和α-酮戊二酸(AKG)对这两种代谢物进行功能验证。在注射食蟹前，分别饲喂纤维二糖(1、5和10 mg/g饲料重量)或AKG(1、5和10 mg/g饲料重量)。结果表明，三种浓度的纤维二糖和AKG均能降低蟹的死亡率，降低血细胞中的埃氏葡萄球菌的拷贝数。中华绒螯蟹后肠的组织病理学切片显示，纤维二糖和AKG影响肌肉外层的结缔组织。总体而言，这些结果为TD控制和水产养殖的可持续发展提供了洞察。
As one of the most devastating diseases in Eriocheir sinensis, tremor disease (TD) is caused by Spiroplasma eriocheiris and dramatically increases the economic losses of crab aquaculture. With the development of biotechnology, metabolomics plays an important role in the screening of metabolites related to physiological and pathological changes. In this study, the different metabolites induced by S. eriocheiris were investigated in hemocytes of crab E. sinensis using metabolomics based on GC/MS among diverse phenotypes groups (tremor group, no-symptom group, and control group). Compared with the tremor group, 42 differential metabolites (26 up-regulated and 16 down-regulated) were significantly different in the no-symptom group. The KEGG pathway enrichment analysis showed that the differential metabolites were enriched in "ABC transporters", "Vitamin B6 metabolism", "Ascorbate and aldarate metabolism" and "Starch and sucrose metabolism". The cellobiose and a-ketoglutarate (AKG) were chosen to verify the function of these two metabolites. The crabs were fed with dietary cellobiose (1, 5, and 10 mg/g diet weight) or AKG (1, 5, and 10 mg/g diet weight) before S. eriocheiris injection. The results show that all three concentrations of cellobiose and AKG could reduce the mortality of crabs and decrease the copies of S. eriocheiris in the hemocytes. The histopathological sections of the hindgut of E. sinensis show that cellobiose and AKG affect the connective tissue of the outer layer of muscle. Overall, these results provide insight into TD control and the sustainable development of aquaculture.