Direct detection of red blood cell fragments: a new flow cytometric method to evaluate hemolysis in blood pumps.
Direct detection of red blood cell fragments: a new flow cytometric method to evaluate hemolysis in blood pumps.
复制标题
直接检测红细胞碎片：一种评估血泵溶血的新流式细胞术方法。
DOI：
10.1097/00002480-200109000-00027
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发表时间：
2000
期刊：
影响因子：
4.2
通讯作者：
Y. Nosé
中科院分区：
文献类型：
--
作者：
J. Linneweber;T. Chow;T. Takano;Tomohiru Maeda;K. Nonaka;S. Schulte;S. Kawahito;O. Elert;J. Moake;Y. Nosé
目前通过测量血浆游离血红蛋白（fHb）来评估泵致溶血。然而，这种方法有缺点，因为fHb的定量取决于红细胞压积（HCT）和血红蛋白（Hb）水平。这项工作的目的是设计一种不依赖于血红蛋白的方法，能够通过测量红细胞（RBC）碎片的数量直接定量细胞损伤。全血流式细胞术用于定量来自滚柱泵（Sarns, Inc.）的循环红细胞片段。型号2m6002)和离心泵（陀螺仪C1E3，京瓷公司）。在模拟回路中对泵进行了2小时（5升/分钟流量，压力扬程为100毫米汞柱）的测试。红细胞片段用红细胞特异性的植红蛋白（PE）标记的糖蛋白a抗体进行定量。红细胞碎片小于完整的红细胞群，并且在大小上与血小板群重叠（基于前向和侧向光散射测量）。对于滚柱泵，RBC碎片值从0分钟时的1090 +/- 260/微升增加到120分钟后的14880 +/- 5900 /微升。相比之下，使用离心泵时，红细胞碎片几乎没有增加（从0分钟时的730 +/- 270/微升到120分钟后的1400 +/- 840/微升）。流式细胞术可用于快速、灵敏、不依赖血红蛋白的泵致红细胞损伤评价。
Pump induced hemolysis is presently evaluated by measuring plasma free hemoglobin (fHb). However, this method has disadvantages because quantification of fHb depends on hematocrit (HCT) and hemoglobin (Hb) levels. The aim of this work was to devise a hemoglobin independent method, capable of quantifying cell trauma directly by measuring the number of red blood cell (RBC) fragments. Whole blood flow cytometry was used to quantify circulating RBC fragments derived from a roller pump (Sarns, Inc. Model 2 M 6,002) and a centrifugal pump (Gyro C1E3, Kyocera Corp.). The pumps were tested in a mock circuit for 2 hr (5 L/min flow against 100 mm Hg pressure head). Red blood cell fragments were quantified by a phycoerythrin (PE) labeled glycophorin A antibody specific for erythrocytes. Red blood cell fragments were smaller than the intact RBC population and overlapped in size with the platelet population (based on forward- and side-light scattering measurements). For the roller pump, the values for RBC fragments increased from 1,090 +/- 260/microl at 0 min to 14,880 +/- 5,900/microl after 120 min. In contrast, using the centrifugal pump, there was little increase in RBC fragments (from 730 +/- 270/microl at 0 min to 1,400 +/- 840/microl after 120 min). Flow cytometry can be used for the rapid, sensitive, hemoglobin independent evaluation of pump induced RBC trauma.