Vasoconstrictor nerve influence on isolated mesenteric resistance vessels from normotensive and spontaneously hypertensive rats.
Vasoconstrictor nerve influence on isolated mesenteric resistance vessels from normotensive and spontaneously hypertensive rats.
复制标题
血管收缩神经对正常血压和自发性高血压大鼠离体肠系膜阻力血管的影响。
DOI：
10.1111/j.1748-1716.1982.tb07131.x
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
B. Folkow
中科院分区：
文献类型：
--
作者：
H. Nilsson;B. Folkow
Methods. Seven SHR and 7 WKY, 12-14 week old males weighing 250-300 g, were used. Small arteries (inner diameters at full relaxation 150-200 pm) from the arcades feeding the jejunum were cautiously dissected free from surrounding tissue. From these vessels, 1.5 mm long segments were threaded onto two fine steel wires and suspended in a myograph, in which their isometric wall tension at well defined internal circumferences was recorded.The myograph (Mulvany & Nyborg 1980) permitted one SHR and one WKY vessel to be studied in parallel in the same organ bath. Initially, the passive tension-diameter relations were determined, and from these the diameter, d, oo, was estimated, which the fully relaxed vessels would have if rounded and subjected to 100 mmHg transmural pressure. The two vessels were then set to 0.9 dlW, where they develop roughly maximal active tension (Mulvany & Halpern 1977). Nerve stimulations were performed by electrical field stimulation. For each vessel segment two electrodes were placed in the bath 2-3 mm from each end. Rectangular constant-current pulses with 2 ms duration were passed between the electrodes. Cur-rent strength was initially adjusted to achieve maximal contractile responses for a submaximal frequency and was mostly 70-85 mA. The responses obtained were well reproducible and were reversibly blocked by tetrodotoxin and by a-blockers (Nilsson & Folkow 1982), thus proving their origin to be neurogenic without any element of direct muscle excitation.
