Digoxin immunoreactivity in cord and maternal serum and placental extracts. Partial characterization of immunoreactive substances by high-performance liquid chromatography and inhibition of Na+, K+-ATPase.
Digoxin immunoreactivity in cord and maternal serum and placental extracts. Partial characterization of immunoreactive substances by high-performance liquid chromatography and inhibition of Na+, K+-ATPase.
复制标题
脐带和母体血清以及胎盘提取物中的地高辛免疫反应性。
DOI：
10.1016/s0009-9120(85)80023-0
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发表时间：
1985
影响因子：
2.8
通讯作者：
S. Soldin
中科院分区：
文献类型：
--
作者：
E. Diamandis;A. Papanastasiou;S. Soldin
已成功地从脐带血清、混合血清（脐带和母体）和胎盘中提取并浓缩了地高辛样免疫反应物质（DLIS）。也从正常成人血清中提取了类似的物质，但这种培养基中的DLIS浓度要低得多。用反相高效液相色谱法将浓缩的DLIS分离成几个免疫活性组分。通过测量红细胞在这些组分存在下的86 Rb摄取，测试免疫反应组分抑制Na−，K+-ATP酶的能力。一个有效的抑制剂中确定的免疫反应性馏分，其中也含有孕酮，但结果表明，泵抑制剂不是孕酮。交叉反应性研究利用荧光偏振免疫分析表明，可的松是最有效的免疫反应性物质的脐带血清。
Digoxin-like immunoreactive substances (DLIS) have been successfully extracted and concentrated from cord serum, mixed (cord and maternal) serum and placentas. Similar substances have also been extracted from normal adult serum, but DLIS in this medium are present in much lower concentrations. Concentrated DLIS have been separated into several immunoreactive fractions by use of reversephase high-performance liquid chromatography. Immunoreactive fractions were tested for their ability to inhibit the Na−, K+-ATPase by measuring the86Rb-uptake of red blood cells in the presence of these fractions. A potent inhibitor was identified in an immunoreactive fraction which also contains progesterone, but the results suggest that the pump inhibitor is not progesterone. Cross-reactivity studies utilising fluorescence polarization immunoassay have shown that cortisone is the most potent immunoreactive substance of cord serum.
利尿钠激素：终于装瓶了？
DOI：
--
发表时间：
1984
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Grantham,JJ;Edwards,RM
通讯作者：
Edwards,RM