Redistribution of the sheep neonatal Fc receptor in the mammary gland around the time of parturition in ewes and its localization in the small intestine of neonatal lambs
Redistribution of the sheep neonatal Fc receptor in the mammary gland around the time of parturition in ewes and its localization in the small intestine of neonatal lambs
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DOI：
10.1046/j.1365-2567.2002.01514.x
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发表时间：
2002-11-01
期刊：
影响因子：
6.4
通讯作者：
Kacskovics, I
中科院分区：
文献类型：
--
作者：
Mayer, B;Zolnai, A;Kacskovics, I
反刍动物的母体免疫完全由初侧免疫球蛋白介导。由于新生儿Fc受体（FcRn）被认为参与了免疫球蛋白G （IgG）在乳腺中的转运，我们从绵羊身上克隆了该受体，并分析了其在分娩前后乳腺和新生羔羊小肠中的表达。FcRn重链mRNA仅在产前和产后乳腺腺泡和导管上皮细胞活检中检测到。免疫组化结果显示，产前乳腺腺泡上皮细胞和乳腺导管细胞质染色均匀。在羔羊后的模式中观察到显着的差异，其中细胞的顶端侧被强烈染色。FcRn存在于乳腺腺泡和导管上皮细胞中，且在分娩前后分布变化明显，说明FcRn在反刍动物初乳形成过程中IgG的转运中起重要作用。免疫组织化学分析在新生羔羊的十二指肠隐窝细胞中检测到强的顶端和弱的基部FcRn信号，这些信号在新生反刍动物中已被证明分泌IgG1。在十二指肠肠细胞中未检测到FcRn，十二指肠肠细胞以非特异性方式从初乳中吸收完整的IgG。这些数据表明FcRn参与了反刍动物上皮细胞IgG1的分泌。
Maternal immunity is mediated exclusively by colostral immunoglobulins in ruminants. As the neonatal Fc receptor (FcRn) is suggested to be involved in the transport of immunoglobulin G (IgG) in the mammary gland, we cloned this receptor from sheep and analysed its expression in the mammary gland around the time of parturition and also in the small intestine from the newborn lamb. FcRn heavy-chain mRNA was detected (by using in situ hybridization) exclusively in the acinar and ductal epithelial cells in mammary gland biopsies both before and after parturition. Immunohistochemistry revealed that the cytoplasm of the epithelial cells of the acini and ducts in the mammary gland biopsies stained homogeneously before parturition. A remarkable difference was observed in the pattern after lambing, where the apical side of the cells was strongly stained. The presence of the FcRn in the acinar and ductal epithelial cells of the mammary gland, and the obvious change in distribution before and after parturition, indicate that the FcRn plays an important role in the transport of IgG during colostrum formation in ruminants. Immunohistochemical analysis detected a strong apical and a weak basal FcRn signal in the duodenal crypt cells of a neonatal lamb, which have been previously demonstrated to secrete IgG1 in newborn ruminants. The FcRn was not detected in the duodenal enterocytes, which absorb intact IgG from the colostrum in a non-specific manner. These data suggest that FcRn is involved in IgG1 secretion in ruminant epithelial cells.