Potent anti-prostate cancer immune response induced by dendritic cells transduced with recombinant adenoviruses encoding 4-1BBL combined with cytokine-induced killer cells
Potent anti-prostate cancer immune response induced by dendritic cells transduced with recombinant adenoviruses encoding 4-1BBL combined with cytokine-induced killer cells
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DOI：
10.2217/imt.14.92
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发表时间：
2015-01-01
期刊：
影响因子：
2.8
通讯作者：
Gou Xin
中科院分区：
文献类型：
--
作者：
Kuang Youlin;Kang Jian;Gou Xin
目的：目的观察腺病毒介导的4-1BBL基因转染的树突状细胞（DC）联合苦参碱诱导的杀伤细胞（CIK）对前列腺癌的治疗作用。方法：采用流式细胞仪检测共培养细胞表面标志物的表达，观察共培养细胞对前列腺癌细胞的体外细胞毒活性和体内抗肿瘤活性。结果如下：结果表明，Ad-4- 1BBL转染的DC可增加CIK细胞中CD 3（+）CD 56（+）细胞的比例，CIK细胞与Ad-4- 1BBL转染的DC共培养可增加CIK细胞分泌IL-12和IFN-γ，降低TGF-β的分泌。此外，Ad-4- 1BBL转导的DC增强了CIK对前列腺癌的细胞毒性，并导致肿瘤生长的抑制和荷瘤动物的存活。结论：4- 1BBL基因修饰的DC能增强CIK细胞对前列腺癌细胞的杀伤活性。
Aim: To test the effect of dendritic cells (DCs) transduced with recombinant adenoviruses encoding 4-1BBL combined with cytokine-induced killer cells (CIKs) against prostate cancer. Method: Flow cytometry was used to detect the surface markers of the co-cultured cells, and cytotoxicity against prostate cancer cells in vitro as well as antitumor activities in vivo were observed. Results: Our results showed that Ad-4-1BBL-transduced DCs could increase percentage of CD3(+)CD56(+) cells in CIKs, and CIKs co-cultured with Ad-4-1BBL-transduced DCs could augment the secretion of IL-12 and IFN-gamma and decrease TGF-beta production. In addition, Ad-4-1BBL-transduced DCs enhanced the cytotoxicity of CIKs against prostate cancer and resulted in inhibition of tumor growth and tumor-bearing animals' survival. Conclusion: These results demonstrate that 4-1BBL-engineered DCs can improve CIKs cytotoxicity against prostate cancer cells.