Cytocidal effects of human fibroblasts on HeLa cells in vitro
Cytocidal effects of human fibroblasts on HeLa cells in vitro
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人成纤维细胞对HeLa细胞的体外杀细胞作用
DOI：
10.1111/j.1768-322x.1987.tb00517.x
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发表时间：
1987
影响因子：
2.7
通讯作者：
J. G. Delinassios
中科院分区：
文献类型：
--
作者：
J. G. Delinassios
体外共培养人成纤维细胞和HeLa细胞导致特定生长模式的发展。这些模式取决于初始混合接种物中的细胞密度和细胞-细胞比。人成纤维细胞在体外共培养超过10天后，不进行传代培养，每2天更换一次培养基，可引起HeLa细胞广泛的核碎裂和细胞解体。这种现象之前的成纤维细胞的人口平行于HeLa集落的边缘和周围的定向运动和过度生长的两种细胞类型在边界网站。因此，在HeLa集落周围形成了密集的边界。在没有每2天重新喂养的情况下，HeLa细胞可以过度生长，通过密集的边界并形成新的区域。在这个阶段再补料可以再次导致在HeLa区周围形成第二个同心致密边界。这种现象可能代表了肿瘤细胞侵袭性的体外隐喻。然而，它也指出了喂养对于激活成纤维细胞对抗HeLa细胞的重要性。
Cocultivation of human fibroblasts and HeLa cells in vitro leads to the development of specific patterns of growth. These patterns depend on the cell density and cell‐cell ratio in the initial mixed inoculum. Human fibroblasts can cause extensive nuclear fragmentation and cellular disintegration of HeLa cells in vitro after coculture for periods longer than 10 days, without subculturing, and with medium replacement every 2 days. This phenomenon is preceded by directional locomotion of the fibroblastic population parallel to the edges of and around HeLa colonies and by overgrowth of both cell types at the border sites. A dense border is thus developed around the HeLa colonies. In the absence of refeeding every 2 days, HeLa cells can overgrow, pass the dense border and form a new zone. Refeeding at this stage can again cause the formation of a second concentric dense border around the HeLa zone. This phenomenon may represent an in vitro metaphor of the invasive property of neoplastic cells. It also points out, however, the importance of feeding for the activation of fibroblasts against HeLa cells.