Activation regions in a yeast transposon have homology to mating type control sequences and to mammalian enhancers.
Activation regions in a yeast transposon have homology to mating type control sequences and to mammalian enhancers.
复制标题
酵母转座子中的激活区与交配型控制序列和哺乳动物增强子具有同源性。
DOI：
10.1073/pnas.82.16.5423
复制
发表时间：
1985
影响因子：
11.1
通讯作者：
Yarnell,WS
中科院分区：
文献类型：
--
作者：
Errede,B;Company,M;Ferchak,JD;Hutchison3rd,CA;Yarnell,WS
提供了来自酿酒酵母CYC 7-H2突变体的Ty 1激活区的DNA序列。对数据的分析揭示了四种猿猴病毒40型增强子核心序列的存在。Ty 1增强子核心中的两个与MAT α处的二倍体控制位点也同源的序列邻接。我们假设这两个Ty 1区域约等于30个碱基对的调控块，我们已经分析了删除，以确定它们是否是必要的Ty 1对相邻基因表达的影响。我们发现，激活丢失时，一个限制性片段，包括两个假定的调控块被删除。删除限制性片段，删除两个调控块之一，有很少或没有影响的Ty 1激活能力在单倍体酵母细胞或抑制这种功能在二倍体酵母细胞。由于分析的限制性片段中最显著的内部同源性是推定的调控块，因此这些观察结果表明，增强子样序列参与Ty 1对基因表达的影响的细胞类型控制。
The DNA sequence of the Ty1 activating region from the CYC7-H2 mutant of Saccharomyces cerevisiae is presented. Analysis of the data revealed the presence of four simian virus 40-type enhancer core sequences. Two of the Ty1 enhancer cores are contiguous with sequences also homologous to the diploid control site at MAT alpha. We postulate that these two Ty1 regions of approximately equal to 30 base pairs are regulatory blocks, and we have analyzed deletions to ascertain whether they are necessary for effects of Ty1 on adjacent gene expression. We found that activation is lost when a restriction fragment encompassing both postulated regulatory blocks is deleted. Deletion of restriction fragments that remove only one of the two regulatory blocks has little or no effect on Ty1 activating ability in haploid yeast cells or on repression of this function in diploid yeast cells. Because the most significant internal homologies in the restriction fragments analyzed are the putative regulatory blocks, these observations suggest that enhancer-like sequences are involved in cell-type control of Ty1 effects on gene expression.