Divergent responses of functional gene expression to various nutrient conditions during microcystin-LR biodegradation by Novosphingobium sp THN1 strain
Divergent responses of functional gene expression to various nutrient conditions during microcystin-LR biodegradation by Novosphingobium sp THN1 strain
复制标题
新鞘藻 THN1 菌株微囊藻毒素-LR 生物降解过程中功能基因表达对不同营养条件的不同反应
DOI：
10.1016/j.biortech.2013.12.118
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发表时间：
2014-03-01
影响因子：
11.4
通讯作者：
Qiao, Yuhui
中科院分区：
文献类型：
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作者：
Li, Jieming;Peng, Liujing;Qiao, Yuhui
为了更好地了解微囊藻毒素-LR（MCLR）的生物降解机制，MCLR的生物降解动力学和功能基因表达动力学之间的联系最初研究与Novosphingobium sp. THN 1作为接种物在各种营养条件下。沿着生物降解，mlrA基因表达，加上mlrD，呈现出类似的趋势，但在不同的条件下调节差异。在相应条件下，直到42 h，MbR降解率与功能基因的诱导率之间均呈良好的正相关。与无营养条件下相比，磷酸氢二钾（DP）或氯化铵（AC）（均为100 mg L-1）对降解的促进或减缓与mlr基因表达的上调或下调有关，表明mlr基因表达的差异是DP或AC对降解作用不同的原因之一。然而，刺激降解与硝酸钠（100毫克L-1）可能涉及其他机制，其中mlr表达是不是决定性的先决条件，以管理MCLR-生物降解。(C)2014爱思唯尔有限公司版权所有。
To better understand the mechanisms for microcystin-LR (MCLR) biodegradation, the linkage between MCLR-biodegradation kinetics and functional gene expression dynamics was originally investigated with Novosphingobium sp. THN1 as inoculum under various nutrient conditions. Along biodegradation, mlrA gene expression, coupled with mlrD, presented similar trend but was regulated differentially among different conditions. Good positive correlation was observed between MCLR degraded and induction ratios of functional genes until 42 h at respective condition. Compared to those under nutrient-free condition, the stimulated or decelerated biodegradation with dipotassium phosphate (DP) or ammonium chloride (AC) (both at 100 mg L-1) was related to higher or lower up-regulation in mlr gene expression, suggesting that divergent mlr gene expression was one of the reasons for different effects of DP or AC on degradation. However, stimulated degradation with sodium nitrate (100 mg L-1) might involve other mechanisms where mlr expression was not the decisive prerequisite to govern MCLR-biodegradation. (C) 2014 Elsevier Ltd. All rights reserved.