The genome sequence analysis of H5N1 avian influenza A virus isolated from the outbreak among poultry populations in Thailand
The genome sequence analysis of H5N1 avian influenza A virus isolated from the outbreak among poultry populations in Thailand
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DOI：
10.1016/j.virol.2004.06.045
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发表时间：
2004-10-25
期刊：
影响因子：
3.7
通讯作者：
Poovorawan, Y
中科院分区：
文献类型：
--
作者：
Viseshakul, N;Thanawongnuwech, R;Poovorawan, Y
在这份报告中……泰国甲型H5N1禽流感病毒基因组；对从2004年初泰国甲型禽流感（AI）流行中分离出来的A/Chicken/Nakom-Pathom/Thailand/CU-K2/04进行了测序。对香港1997年爆发的禽流感病毒和2001-2004年报告的其他禽流感（H5N1）分离株进行了系统发育分析。泰国禽流感（H5N1） HA基因的分子特征揭示了高致病性禽流感（HPAI）的共同特征，神经氨酸酶基因20个密码子缺失，NS基因5个密码子缺失，M2和PB2基因多态性。此外，泰国禽流感病毒的HA和NA基因与同一次疫情中从人类病例中分离的禽流感病毒的基因高度相似。最后，我们的研究结果表明，泰国禽流感是2000年禽流感谱系的成员，其大部分基因序列与甲型流感/鸭/中国/E319.2/03 （H5N1）密切相关。(C) 2004爱思唯尔公司版权所有。
In this report.. the genome of the Thai avian influenza virus A (H5N1); A/Chicken/Nakom-Pathom/Thailand/CU-K2/04, isolated from the Thai avian influenza A (AI) epidemic during the early of 2004 was sequenced. Phylogenetic analyses were performed in comparison to AI viruses from Hong Kong 1997 outbreaks and other AI (H5N1) isolates reported during 2001-2004. Molecular characterization of the Thai AI (H5N1) HA gene revealed a common characteristic of a highly pathogenic AI (HPAI), a 20-codon deletion in the neuraminidase gene, a 5-codon deletion in the NS gene and polymorphisms of the M2 and PB2 genes. Moreover, the HA and NA genes of the Thai AI displayed high similarity to those of the AI viruses isolated from human cases during the same epidemic. Finally, our results demonstrated that the Thai AI emerged as a member of 2000's AI lineage with most of the genetic sequences closely related to the Influenza A/Duck/China/E319.2/03 (H5N1). (C) 2004 Elsevier Inc. All rights reserved.