Both the conserved GRAS domain and nuclear localization are required for SHORT-ROOT movement.
Both the conserved GRAS domain and nuclear localization are required for SHORT-ROOT movement.
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DOI：
10.1111/j.1365-313x.2008.03735.x
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发表时间：
2009-03
期刊：
影响因子：
--
通讯作者：
Benfey PN
中科院分区：
文献类型：
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作者：
Gallagher KL;Benfey PN
转录因子的运动在植物中已经很成熟。自从首次报道 KNOTTED 运动以来，已有十多个转录因子被证明可以在植物中运动。然而，运动的发育意义尚不清楚。使用短根（SHR）转录因子作为研究细胞间运输的工具，我们表明SHR从其合成位点的移动对于拟南芥根的正常发育是必要的。我们确定了 SHR 细胞内和细胞间运动所需的多个 SHR 区域，包括运动但非活动所需的区域。我们惊奇地发现，细胞间运动的能力在其他 GRAS 家族蛋白中可能是保守的。最后，我们提供的证据表明运动需要细胞质和核定位，强烈表明核运输和细胞间运动之间存在机械联系。
Transcription factor movement is well established in plants. Since the initial report of KNOTTED movement, more than a dozen transcription factors have been shown to move in plants. However, the developmental significance of movement is not known. Using the SHORT-ROOT (SHR) transcription factor as a tool for studying cell-to-cell trafficking, we show that movement of SHR from its site of synthesis is necessary for normal development of the Arabidopsis root. We identify multiple regions of SHR that are required for intra-and intercellular movement of SHR, including a region that is necessary for movement but not activity. We made the surprising discovery that the capacity for intercellular movement may be conserved among other GRAS family proteins. Finally, we provide evidence that movement requires both cytoplasmic and nuclear localization, strongly suggesting a mechanistic link between nuclear transport and cell-to-cell movement.