Comparison of cytotoxic and inflammatory responses of pristine and functionalized multi-walled carbon nanotubes in RAW 264.7 mouse macrophages
Comparison of cytotoxic and inflammatory responses of pristine and functionalized multi-walled carbon nanotubes in RAW 264.7 mouse macrophages
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原始和功能化多壁碳纳米管在 RAW 264.7 小鼠巨噬细胞中的细胞毒性和炎症反应的比较
DOI：
10.1016/j.jhazmat.2012.03.079
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发表时间：
2012-06-15
影响因子：
13.6
通讯作者：
Pu, Yuepu
中科院分区：
文献类型：
--
作者：
Zhang, Ting;Tang, Meng;Pu, Yuepu
碳纳米管（CNT）应用的增加提高了公众对可能的毒性的关注程度。使用体外细胞试验，我们能够评估原始多壁碳纳米管（MWCNTs）及其衍生物，共价官能化与羧基（COOH）或聚乙二醇（PEG），在啮齿动物巨噬细胞的免疫毒性。此外，特别关注的作用，表面改性和纳米管的毒性聚集。结果表明，原始的多壁碳纳米管降低细胞活力相比，功能化的多壁碳纳米管在RAW 264.7巨噬细胞在25，50，100，200，400，和800 μ g/mL的浓度孵育。然而，除了引起细胞毒性之外，官能化的MWCNT诱导严重的炎症反应，如通过在各种MWCNT浓度（25、50、100和200 μ g/mL）下产生包括TNF-α、IL-1 β和IL-6的炎症细胞因子所指示的。颗粒表面修饰和在生物介质中的分散状态是决定细胞毒性的关键因素。这些发现意味着，多壁碳纳米管诱导的巨噬细胞的炎症反应可能与表面修饰和聚集的多壁碳纳米管，这是通过改变炎症细胞因子的表达。(C)2012爱思唯尔有限公司版权所有。
The increased application of carbon nanotubes (CNTs) has raised the level of public concern regarding possible toxicities. Using in vitro cellular assays, we were able to assess the immunotoxicity of pristine multi-wall carbon nanotubes (MWCNTs) and their derivatives, covalently functionalized with carboxyl (COOH) or polyethylene glycol (PEG), in rodent macrophage cells. Moreover, special focus was placed on the role of surface modification and nanotubes aggregation on toxicity. Results showed that pristine MWCNTs reduce cell viability compared with functionalized MWCNTs in RAW 264.7 macrophages when incubated at concentrations of 25, 50, 100, 200, 400, and 800 mu g/mL. However, in addition to causing cytotoxicity, functionalized MWCNTs induce serious inflammatory responses, as indicated by the production of inflammatory cytokines including TNF-alpha, IL-1 beta and IL-6 at various MWCNTs concentrations (25, 50, 100, and 200 mu g/mL). Particle surface modification and dispersion status in biological medium were key factors in determining cytotoxicity. These findings imply that MWCNTs-induced inflammatory responses in macrophages may be associated with surface modification and aggregation of MWCNTs, which is reflected by alteration of inflammatory cytokine expression. (C) 2012 Elsevier B.V. All rights reserved.