Tissue Deposition and Residue Depletion in Laying Hens Exposed to Melamine-Contaminated Diets
Tissue Deposition and Residue Depletion in Laying Hens Exposed to Melamine-Contaminated Diets
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DOI：
10.1021/jf904026n
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发表时间：
2010-05-12
影响因子：
6.1
通讯作者：
Lin, Shunqin
中科院分区：
文献类型：
--
作者：
Bai, Xue;Bai, Fan;Lin, Shunqin
为了研究三聚氰胺在母鸡鸡蛋和组织中的沉积和消除，对72只罗马产蛋鸡进行了为期34天的三聚氰胺注射，剂量为每公斤体重8.6-140.9毫克/天。在三聚氰胺含量为62.6毫克或140.9毫克/公斤的母鸡的三个肾脏中，有一个肾脏中发现了这些晶体。采用高效液相色谱-紫外（HPLC-UV）法测定鸡蛋、肌肉、肝脏、肾脏、胃、十二指肠、子宫、卵巢和血浆中的三聚氰胺浓度。饮食中三聚氰胺的含量越高，组织和鸡蛋中的三聚氰胺含量就越高。组织中三聚氰胺的浓度范围（μ g/g）：鸡蛋，1.1 ~ 28.7；肌肉,0.4 - -9.3;肝脏,0.5 - -6.9;肾脏,1.3 - -21.7;胃,0.4 - -7.3;十二指肠0 3 - 2.8;子宫,0.5 - -6.9;卵巢,0.5 - -9.1;血浆，0.8-7.6。从日粮中剔除三聚氰胺后，第10天母鸡组织中的三聚氰胺浓度降至2.5 μ g/g以下，第7天和第20天鸡蛋和组织中均未检测到三聚氰胺残留。
To investigate the deposition and elimination of melamine in hen eggs and tissues, 72 Roman laying hens were administrated with melamine at 8.6-140.9 mg per kilogram of body weight per day for 34 days. The crystals were found in one of three kidneys of hens treated with melamine at either 62.6 or 140.9 mg/kg. Furthermore, the melamine concentrations in egg, muscle, liver, kidney, stomach, duodenum, uterus, ovary, and blood plasma were determined by high-performance liquid chromatography ultraviolet (HPLC-UV) methods. A higher dosage of melamine in the diet corresponded to higher concentrations in tissues and eggs. The concentrations of melamine in tissues were in the following ranges (mu g/g): egg, 1.1-28.7; muscle, 0.4-9.3; liver, 0.5-6.9; kidney, 1.3-21.7; stomach, 0.4-7.3; duodenum, 0,3-2.8; uterus, 0.5-6.9; ovary, 0.5-9.1; and blood plasma, 0.8-7.6. When melamine was withdrawn from the diet of hens, the melamine concentration in hen tissues fell to below 2.5 mu g/g by day 10 and no residues were detected in eggs or tissues at days 7 and 20, respectively.