Analysis of cell movement and signalling during ring formation in an activated G alpha1 mutant of Dictyostelium discoideum that is defective in prestalk zone formation.
Analysis of cell movement and signalling during ring formation in an activated G alpha1 mutant of Dictyostelium discoideum that is defective in prestalk zone formation.
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分析盘基网柄菌激活的 G α1 突变体环形成过程中的细胞运动和信号传导，该突变体在前柄区形成方面存在缺陷。
DOI：
10.1006/dbio.1996.8447
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Weijer,CJ
中科院分区：
文献类型：
--
作者：
Rietdorf,J;Siegert,F;Dharmawardhane,S;Firtel,RA;Weijer,CJ
细胞黏菌中的丘状结构是由感态细胞的趋化聚集形成的。周期性cAMP信号以多臂螺旋波的形式传播，协调细胞的运动。在聚集体后期，细胞分化为前孢子和几种前柄细胞类型。前柄细胞通过化学反应形成尖端，然后控制所有进一步的发育。尖端通过涡旋波组织细胞运动，涡旋波在孢子前区转化为平面波，导致细胞在尖端旋转运动，并在孢子前区周期性向前运动。激活的Gα1蛋白在其自身启动子下的表达导致从丘期开始的形态发生严重改变。细胞不是形成一个有尖的丘，而是形成一个没有尖的环状结构。突变体在聚集和丘形成过程中的波传播模式和动力学与亲本菌株AX3难以区分。然而，在叶尖形成时，组织后期聚集体的螺旋波并不是在叶尖的涡旋组织中心演化，而是转变为圆形封闭（扭曲）的涡旋环波。这导致形成一个甜甜圈形状的聚集体。在进一步的发展过程中，甜甜圈的直径增加，扭曲的涡旋波转化为一列平面波，导致周期性的旋转细胞运动。虽然这种突变导致的生化后果尚不清楚，但它一定会影响柄前细胞的分化。突变体产生正常比例的前孢子细胞，但不能形成功能性的前柄细胞，即不能形成能够从前孢子细胞中分选出来并形成前柄区的前柄细胞。如果分选失败，信号的几何形状就会发生改变，形成环状的涡旋波，从而导致环形的形成。这个突变体证明了茎前细胞分选对组织尖端的涡旋波稳定的重要性。
Mound formation in the cellular slime mouldDictyosteliumresults from the chemotactic aggregation of competent cells. Periodic cAMP signals propagate as multiarmed spiral waves and coordinate the movement of the cells. In the late aggregate stage the cells differentiate into prespore and several prestalk cell types. Prestalk cells sort out chemotactically to form the tip, which then controls all further development. The tip organises cell movement via a scroll wave that converts to planar waves in the prespore zone leading to rotational cell movement in the tip and periodic forward movement in the prespore zone. Expression of an activated Gα1 protein under its own promoter leads to a severely altered morphogenesis from the mound stage onwards. Instead of forming a tipped mound, the cells form a ring-shaped structure without tip. Wave propagation pattern and dynamics during aggregation and mound formation in the mutant are indistinguishable from the parental strain AX3. However, at the time of tip formation the spiral waves that organise the late aggregate do not evolve in a scroll-organising centre in the tip but transform into a circularly closed (twisted) scroll ring wave. This leads to the formation of a doughnut-shaped aggregate. During further development, the doughnut increases in diameter and the twisted scroll wave converts into a train of planar waves, resulting in periodic rotational cell movement. Although biochemical consequences resulting from this mutation are still unclear, it must affect prestalk cell differentiation. The mutant produces the normal proportion of prespore cells but is unable to form functional prestalk cells, i.e., prestalk cells with an ability to sort out from the prespore cells and form a prestalk zone. Failure of sorting leads to an altered signal geometry, ring-shaped scroll waves, that then directs ring formation. This mutant demonstrates the importance of prestalk cell sorting for the stabilisation of the scroll wave that organises the tip.
盘基网柄菌中的 GFP。
影响因子：
11.4
作者：
S. Hodgkinson
通讯作者：
S. Hodgkinson
G 蛋白相关信号通路介导盘基网柄菌的发育。
DOI：
10.1101/sqb.1992.057.01.022
发表时间：
1992
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Johnson,RL;Gundersen,R;Hereld,D;Pitt,GS;Tugendreich,S;Saxe,CL;Kimmel,AR;Devreotes,PN
通讯作者：
Devreotes,PN
G α 1 亚基在控制盘基网柄菌细胞形态发生中的调节作用。
DOI：
10.1242/dev.120.12.3549
发表时间：
1994
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Dharmawardhane,S;Cubitt,AB;Clark,AM;Firtel,RA
通讯作者：
Firtel,RA