Induction of 7-ethoxycoumarin o-deethylase activity in cultured human epithelial cells by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD): evidence for TCDD receptor.
Induction of 7-ethoxycoumarin o-deethylase activity in cultured human epithelial cells by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD): evidence for TCDD receptor.
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2,3,7,8-四氯二苯并-对二恶英 (TCDD) 在培养的人上皮细胞中诱导 7-乙氧基香豆素邻脱乙基酶活性：TCDD 受体的证据。
DOI：
10.1016/s0006-291x(83)80188-0
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发表时间：
1983
影响因子：
3.1
通讯作者：
Greenlee,WF
中科院分区：
文献类型：
--
作者：
Hudson,LG;Shaikh,R;ToscanoJr,WA;Greenlee,WF
用细胞色素P1-450介导的单加氧酶7-乙氧基香豆素O-脱乙基酶（ECOD）的诱导作用，研究了2，3，7，8-四氯二苯并-对-二恶英（TCDD）对5株人表皮和舌鳞癌细胞系的反应性。在4个SCC细胞系中，该反应的EC 502约为10- 9 M，而在一个细胞系中，EC 502为10- 10 M。在每一个敏感性较低的线，浓度为10- 10 M的TCDD引起的最大酶活性的5%以下。放射性标记的TCDD的特异性结合检测在所有的SCC线的细胞质组分。在每一行中测量的受体的相对量与最大诱导的ECOD活性相关。这些数据表明，来自TCDD毒性靶组织的人类细胞系含有TCDD受体，并显示出对TCDD的不同敏感性，类似于由Ahlocus调节的小鼠品系敏感性差异。
The responsiveness of 5 human squamous cell carcinoma (SCC)1lines derived from tumors of the epidermis and tongue to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was assessed by measuring the induction of the cytochrome P1-450-mediated monooxygenase activity, 7-ethoxycoumarin O-deethylase (ECOD). In 4 of the SCC lines the EC502for this response was approximately 10-9M, whereas in one line the EC50was 10-10M. In each of the less sensitive lines a concentration of 10-10M TCDD elicited less than 5% of the maximal enzyme activity. Specific binding of radiolabeled TCDD was detected in the cytosol fraction from all the SCC lines. The relative amount of receptor measured in each line correlated with maximally-induced ECOD activity. The data indicate that human cell lines derived from a target tissue for TCDD toxicity contain the TCDD receptor and show differential sensitivity to TCDD analogous to the murine strain differences in sensitivity regulated by theAhlocus.