DISTRIBUTION OF PROFILIN IN FIBROBLASTS CORRELATES WITH THE PRESENCE OF HIGHLY DYNAMIC ACTIN-FILAMENTS
DISTRIBUTION OF PROFILIN IN FIBROBLASTS CORRELATES WITH THE PRESENCE OF HIGHLY DYNAMIC ACTIN-FILAMENTS
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DOI：
10.1002/cm.970220106
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发表时间：
1992-01-01
影响因子：
--
通讯作者：
JOCKUSCH, BM
中科院分区：
文献类型：
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作者：
BUSS, F;TEMMGROVE, C;JOCKUSCH, BM
我们已经使用了多克隆和单克隆抗体提出了对小牛胸腺profilin本地化相应的蛋白质在易位，传播，和静止的大鼠成纤维细胞。 免疫荧光的全细胞和免疫金标记的腹侧膜的裂解喷出的细胞表明，profilin显着富集在高度动态的lamellipodia或伪足叶。 在这些区域内，一个显着的分数是共定位与动态肌动蛋白丝组织在肌动蛋白肋，皮质丝，或应力纤维样束，和小profilin被发现在膜区出现免费的肌动蛋白。 相反，静止细胞的应力纤维以及伸展和迁移细胞的肌动蛋白弧和环状束显示很少的标签。 这些结果在提出的profilin在成纤维细胞典型的区域膜动力学中的作用的背景下进行了讨论，并与以前的数据进行了比较（Hartwig等人： 109：1571-1579，1989）对血小板和粒细胞中的profilin分布的研究。
We have used polyclonal and monoclonal antibodies raised against calf thymus profilin to localize the corresponding protein in translocating, spreading, and stationary rat fibroblasts. Immunofluorescence of whole cells and immunogold labeling on ventral membranes of lysis-squirted cells showed that profilin was markedly enriched in the highly dynamic lamellipodia or pseudopodial lobes. Within these regions, a significant fraction was colocalized with dynamic actin filaments organized in actin ribs, cortical filaments, or stress fiber-like bundles, and little profilin was found in membrane areas appearing free of actin. In contrast, stress fibers of stationary cells as well as actin arcs and ring-like bundles of spreading and migrating cells showed very little label. These results are discussed in context with the proposed role of profilin in regional membrane dynamics typical for fibroblasts and are compared to previous data (Hartwig et al.: J. Cell Biol. 109:1571-1579, 1989) on profilin distribution in platelets and granulocytes.