Thermal tolerance of a thermally selected strain of rainbow trout Oncorhynchus mykiss and the pedigrees of its F1 and F2 generations indicated by their critical thermal maxima
Thermal tolerance of a thermally selected strain of rainbow trout Oncorhynchus mykiss and the pedigrees of its F1 and F2 generations indicated by their critical thermal maxima
复制标题
虹鳟鱼 Oncorhynchus mykiss 的热选择品系的热耐受性及其 F1 和 F2 代谱系的临界热最大值表明
DOI：
10.1007/s12562-018-1217-2
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发表时间：
2018
影响因子：
1.9
通讯作者：
Watabe Shugo
中科院分区：
文献类型：
--
作者：
Ineno Toshinao;Tamaki Koichi;Yamada Kazuya;Kodama Ryusuke;Tsuchida Shuji;Tan Engkong;Kinoshita Shigeharu;Muto Koji;Yada Takashi;Kitamura Shoji;Asakawa Shuichi;Watabe Shugo
自 1966 年以来，日本宫崎通过选育培育出热选虹鳟鱼品种。在本研究中，我们比较了热选择虹鳟鱼和两种正常（唐纳森）虹鳟鱼品系之间的临界最高温度（CTM），即生物体达到预定亚致死终点并失去平衡的温度。适应 20 °C (29.7 °C) 的一种来自 Nikko 的正常菌株（Nikko 菌株）的 CTM 显着低于热选择菌株（30.0 °C）和另一种来自 Aomori 的 Donaldson 菌株（29.9 °C）（P< 0.05）。 F1代F1T和F1N分别通过热选择品系雌性与Nikko品系雄性杂交以及Nikko品系雌性与热选择品系雄性杂交产生。对于适应20°C的鱼，F1T [30.1±±0.15°C (n= 30)]和F1N [30.1± 0.16°C (n= 30)]之间的CTM没有观察到显着差异（P> 0.05），这表明F1后代继承了热选择的耐热性状应变父母，无论是男性还是女性。 F1T或F1N的F2后代也表现出耐热性状。还比较了本研究获得的所有数据集的 CTM 变异系数，F1 杂种的变异系数低于 Nikko 品系亲代的变异系数（P<0.05）。相比之下，F2 的变异系数与其亲代相同。此外，热选品系和 Nikko 品系作为参考家系提供了用于分离表型的 F2 代，这是对热选虹鳟鱼品系进行深入遗传分析所必需的。
A thermally selected strain of rainbow trout has been established by selective breeding since 1966 in Miyazaki, Japan. In the present study, we compared the critical thermal maxima (CTMs), the temperatures at which organisms reach a predefined sublethal endpoint and lose their equilibrium, between a thermally selected and two normal (Donaldson) strains of rainbow trout. The CTM of one normal strain from Nikko (Nikko strain) acclimated to 20 °C (29.7 °C) was significantly lower than those of the thermally selected strain (30.0 °C) and the other Donaldson strain from Aomori (29.9 °C) (P< 0.05). The F1 generations, F1T and F1N, were produced by crossing thermally selected strain females with Nikko strain males and Nikko strain females with thermally selected strain males, respectively. No significant difference was observed in the CTM between F1T [30.1 ± 0.15 °C (n= 30)] and F1N [30.1 ± 0.16 °C (n= 30)] (P> 0.05) for fish acclimated to 20 °C, suggesting that the F1 offspring inherited the thermal tolerance trait from one thermally selected strain parent irrespective of whether it was the male or female. F2 offspring of F1T or F1N also showed the thermal tolerance trait. The coefficients of variation for CTM were also compared among all the datasets obtained in the present study and their values for F1 hybrids were lower than those of the parental generation of the Nikko strain (P< 0.05). In contrast, the coefficients of variation of F2s were the same as those of their parental generation. Furthermore, the thermally selected strain and Nikko strain as a reference family provide a F2 generation for segregating phenotypes, which is required for in-depth genetic analysis of the thermally selected rainbow trout strain.