Circular dichroism of heterochromophoric and partially regenerated purple membrane: search for exciton coupling.
Circular dichroism of heterochromophoric and partially regenerated purple membrane: search for exciton coupling.
复制标题
异色团和部分再生紫色膜的圆二色性：寻找激子耦合。
DOI：
10.1002/chir.20222
复制
发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Borhan,Babak
中科院分区：
文献类型：
--
作者：
Karnaukhova,Elena;Vasileiou,Chrysoula;Wang,Andy;Berova,Nina;Nakanishi,Koji;Borhan,Babak
为了确定天然紫膜的双信号CD光谱的来源，研究了用天然全反式视黄醇和视黄醇类似物再生的含有细菌视紫红质的异发色类似物。含有两种不同发色团的紫膜样品收集的数据表明CD光谱的加性特征。该结论得到了使用5，6-二氢视黄醛和3-脱氢视黄醛以及使用缓冲液中33%再生PM和渗透剂的一系列光谱的支持。我们的研究结果支持三聚体中bR中发色团构象异质性的想法，表明三聚体中的三个bR亚基在构象上不相等，因此，紫膜中bR的双信号CD光谱的出现是由于三聚体中各种扭曲的bR亚基的CD光谱的叠加，而不是发色团间的激子耦合相互作用。© 2005 Wiley利斯公司，手性18：72-83，2006年。
In order to determine the origin of the bisignate CD spectra of native purple membrane, heterochromophoric analogues containing bacteriorhodopsin regenerated with nativeall‐trans‐retinal and retinal analogues were investigated. The data collected for the purple membrane samples containing two different chromophores suggest the additive character of the CD spectra. This conclusion was supported by a series of spectra using 5,6‐dihydroretinal and 3‐dehydroretinal and by using 33% regenerated PM in buffer and in presence of osmolytes. Our results support the idea of conformational heterogeneity of the chromophores in the bR in the trimer, suggesting that the three bR subunits in the trimer are not conformationally equal, and therefore, the bisignate CD spectrum of bR in the purple membrane occurs rather due to a superposition of the CD spectra from variously distorted bR subunits in the trimer than interchromophoric exciton‐coupling interactions. © 2005 Wiley‐Liss, Inc.Chirality 18:72–83, 2006.