Imaging green fluorescent protein fusions in living fission yeast cells
Imaging green fluorescent protein fusions in living fission yeast cells
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DOI：
10.1016/j.ymeth.2003.11.017
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发表时间：
2004-07-01
期刊：
影响因子：
4.8
通讯作者：
Chang, F
中科院分区：
文献类型：
--
作者：
Tran, PT;Paoletti, A;Chang, F
使用绿色荧光蛋白 (GFP) 融合物作为生物传感器来检查蛋白质定位和动力学已经彻底改变了细胞生物学。在这里，我们描述了使用荧光显微镜对裂殖酵母裂殖酵母中 GFP 融合进行成像的方法，重点是使用延时成像来分析微管的动态。我们讨论荧光显微镜、细胞制备中的注意事项。适合活细胞分析的数据采集和图像分析。 (C) 2003 Elsevier Inc. 保留所有权利。
The use of green fluorescent protein (GFP) fusions as biosensors for examining protein localization and dynamics has revolutionized cell biology. Here, we describe the methods developed for imaging of GFP-fusions in the fission yeast Schizosaccharomyces pombe using fluorescence microscopy, with a focus on the use of time-lapse imaging to analyze the dynamics of microtubules. We discuss the considerations in fluorescence microscopy, cell preparation. data acquisition, and image analysis appropriate for analysis of living cells. (C) 2003 Elsevier Inc. All rights reserved.