Lactococcus nasutitermitis sp. nov. isolated from a termite gut.
Lactococcus nasutitermitis sp. nov. isolated from a termite gut.
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DOI：
10.1099/ijsem.0.000743
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发表时间：
2016
影响因子：
2.8
通讯作者：
Shu Yan Yang;Ying-Bin Zheng;Zhou Huang;Xue Min Wang;Hong Yang
中科院分区：
文献类型：
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作者：
Shu Yan Yang;Ying-Bin Zheng;Zhou Huang;Xue Min Wang;Hong Yang
从食木白蚁(Nasutitermes Hainanensis)的肠道中分离到细菌M19T。基于16S rRNA基因序列的系统发育分析表明，菌株M19T与乳球菌属成员有亲缘关系，序列相似性在84.8%~95.5%之间。看家基因ropB的序列比较表明，菌株M19T与藤芝乳球菌JCM 16395T和多毛乳球菌117T分离良好。分离株革兰氏阳性，过氧化氢酶阴性，不能运动。细胞呈球形或卵圆形，呈单细胞、成对或短链分布。菌株M19T的生长温度为10~40℃，pH为5.0~7.5。菌株M19T的DNAG+C含量为39.6mol%，主要脂肪酸为C16：0，Cclo-C19：0ω8c，C18：1ω9c，综合特征7和综合特征8。根据系统发育、化学分类和表型数据，菌株M19T代表了乳球菌属的一个新种，命名为Lacto价鼻翼乳球菌。11月是提议的。模式菌株为M19T(=CGMCC 1.15204T=NBRC 111537T)。
Bacterial strain M19T was isolated from the gut of a wood-feeding termite, Nasutitermes hainanensis. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M19T was related to members of the genus Lactococcus, with sequence similarities ranging from 84.8 to 95.5 %. Comparison of housekeeping gene ropB sequences revealed that strain M19T was well separated from Lactococcus fujiensis JCM 16395T and Lactococcus hircilactis 117T. The isolate was Gram-stain-positive, catalase-negative and non-motile. Cells were coccoid or ovoid-shaped, and occurred singly, in pairs or as short chains. Growth of strain M19T occurred at 10-40 °C and at pH 5.0-7.5. The DNA G+C content of strain M19T was 39.6 mol% and the major fatty acids were C16 : 0, cyclo-C19 : 0ω8c, C18 : 1ω9c, summed feature 7 and summed feature 8. Based on the phylogenetic, chemotaxonomic and phenotypic data presented, strain M19T represents a novel species of the genus Lactococcus, for which the name Lactococcus nasutitermitis sp. nov. is proposed. The type strain is M19T ( = CGMCC 1.15204T = NBRC 111537T).