Analysis of changes in relative elemental growth rate patterns in the elongation zone of Arabidopsis roots upon gravistimulation
Analysis of changes in relative elemental growth rate patterns in the elongation zone of Arabidopsis roots upon gravistimulation
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DOI：
10.1007/s004250050437
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发表时间：
1998-11-01
期刊：
影响因子：
4.3
通讯作者：
Evans, ML
中科院分区：
文献类型：
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作者：
Mullen, JL;Ishikawa, H;Evans, ML
虽然拟南芥是研究根系生理的重要系统，但其根的局部生长模式尚未得到很好的定义，特别是在热带反应期间。为了研究拟南芥（Arabidopsis thaliana（L.））初生根顶端沿着生长速率的分布特征，对初生根的生长速率进行了测定。Heynh（生态型哥伦比亚），我们将小木炭颗粒施加到根表面，并使用具有用于跟踪标记的定制软件的自动视频数字化仪系统分析它们在生长期间的位移。垂直生长时，最大伸长速率出现在距根尖481 ± 50 μ m处（根冠顶端），延伸区延伸回到912 +/-137 μ m。远端延伸区（DEZ）先前已被描述为延伸区的顶端区域，其中相对元素生长速率（REGR）小于或等于30%。在中央延伸区的峰值速率。根据这个定义，我们的数据表明，DEZ的基底极限位于距根尖248 +/- 30 pm处。而重力刺激后，根的生长方式发生了变化。在第一个小时内的重力反应，基础限制的DEZ和峰值RECR的位置转移epically上的上侧面的根。这是由于DEZ中的生长增加和中心延伸区中的生长抑制的组合。在下胁腹，DEZ的基底界限随着REGR的降低而向基瓣移动。这些因素建立了根部的生长速率梯度，从而驱动曲率。
Although Arabidopsis is an important system for studying root physiology, the localized growth patterns of its roots have not been well defined, particularly during tropic responses. In order to characterize growth rate profiles along the apex of primary roots of Arabidopsis thaliana (L.) Heynh (ecotype Columbia) we applied small charcoal particles to the root surface and analyzed their displacement during growth using an automated video digitizer system with custom software for tracking the markers. When growing vertically, the maximum elongation rate occurred 481 +/- 50 mu m back from the extreme tip of the root (tip of root cap), and the elongation zone extended back to 912 +/- 137 mu m The distal elongation zone (DEZ) has previously been described as the apical region of the elongation zone in which the relative elemental growth rate (REGR) is less than or equal to 30% of the peak rate in the central elongation zone. By this definition, our data indicate that the basal limit of the DEZ was located 248 +/- 30 pm from the root tip. However, after gravi-stimulation stimulation, the growth patterns of the root changed. Within the first hour of graviresponse, the basal limit of the DEZ and the position of peak RECR shifted epically on the upper flank of the root. This was due to a combination of increased growth in the DEZ and growth inhibition in the central elongation zone. On the lower flank, the basal limit of the DEZ shifted basipetally as the REGR decreased. These factors set up the gradient of growth rate across the root, which drives curvature.