Measurement of Deubiquitinating Enzyme Activity Via a Suicidal HA-Ub-VS Probe
Measurement of Deubiquitinating Enzyme Activity Via a Suicidal HA-Ub-VS Probe
复制标题
DOI：
10.1007/978-1-4939-2013-6_14
复制
发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Bazzaro, Martina
中科院分区：
文献类型：
--
作者：
Rivard, Colleen;Bazzaro, Martina
去泛素化酶（DUBs）是宫颈癌的一个新的“可药物化”靶点，DUBs的小分子抑制剂目前正被评估为新的化疗药物。在这一章中，我们描述了一种酶活性测定，以评估一个假定的小分子DUB抑制剂对癌细胞系中DUB亚组的选择性。
Deubiquitinating enzymes (DUBs) are a novel "drug-able" target for cervical cancer, and small-molecule inhibitors of DUBs are currently being evaluated as novel chemotherapeutic agents. In this chapter, we describe an enzyme activity assay to assess the selectivity of a putative small-molecule DUB inhibitor toward a subset of DUBs in cancer cell lines.