Factors influencing the trailing endpoint observed in Candida albicans susceptibility testing using the CLSI procedure
Factors influencing the trailing endpoint observed in Candida albicans susceptibility testing using the CLSI procedure
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DOI：
10.1111/j.1469-0691.2008.01956.x
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发表时间：
2008-05-01
影响因子：
14.2
通讯作者：
Nelis, H.
中科院分区：
文献类型：
--
作者：
Coenye, T.;De Vos, M.;Nelis, H.
根据CLSI程序，在白色念珠菌对唑类药物敏感性试验期间观察到的拖尾终点表型定义为MIC差异，具体取决于结果是在24小时还是48小时后获得。本研究调查了EUCAST和CLSI方法之间的内在差异是否可以解释拖尾生长。发现培养基中的葡萄糖浓度和微量滴定板威尔斯孔的形状都与此有关。根据CLSI程序，为了减少拖尾生长的发生率，使用更高的葡萄糖浓度和平底微量滴定板可能是有价值的改进。
The trailing endpoint phenotype observed during testing of Candida albicans susceptibility to azoles according to the CLSI procedure is defined as a difference in MIC depending on whether the result is obtained after 24 or 48 h. This study investigated whether intrinsic differences between the EUCAST and CLSI methods could explain trailing growth. The glucose concentration in the medium and the shape of the microtitre plate wells were both found to be involved. In order to reduce the incidence of trailing growth according to the CLSI procedure, the use of higher glucose concentrations and flat-bottomed microtitre plates could be valuable improvements.