Transient receptor potential (TRPC) channels in human sperm:: expression, cellular localization and involvement in the regulation of flagellar motility
Transient receptor potential (TRPC) channels in human sperm:: expression, cellular localization and involvement in the regulation of flagellar motility
复制标题
DOI：
10.1016/s0014-5793(03)00305-3
复制
发表时间：
2003-04-24
期刊：
影响因子：
3.5
通讯作者：
Darszon, A
中科院分区：
文献类型：
--
作者：
Castellano, LE;Treviño, CL;Darszon, A
容量性Ca（2+）内流是细胞内Ca（2+）储存耗尽而触发Ca（2+）通过质膜内流的激活的过程。一些瞬时受体电位（TRPC）蛋白已被提出作为电容性Ca（2+）通道的候选者。最近的证据表明，能性Ca（2+）内流参与精子顶体反应（AR），这是受精所必需的胞吐过程。此外，几个TRPC已被检测到不均匀分布在小鼠精子，这表明他们可能参与其他功能，如运动。用逆转录-聚合酶链反应（RT-PCR）分析，TRPC 1，3，6和7的RNA信使发现在人类生精细胞。共聚焦间接免疫荧光显示存在TRPC 1，3，4和6差异定位在人类精子，和免疫金透射电子显微镜表明，TRPC表位大多与细胞的表面。由于所有这些都在鞭毛中检测到，因此使用计算机辅助测定法测试TRPC通道拮抗剂在精子活力中的作用。我们的研究结果提供了什么是我们所知的第一个证据，这些渠道可能会影响人类精子活力。(C)2003年由Elsevier Science B. V.代表欧洲生物化学学会联合会出版。
Capacitative Ca(2+) entry is a process whereby the activation of Ca(2+) influx through the plasma membrane is triggered by depletion of intracellular Ca(2+) stores. Some transient receptor potential (TRPC) proteins have been proposed as candidates for capacitative Ca(2+) channels. Recent evidence indicates that capacitative Ca(2+) entry participates in the sperm acrosome reaction (AR), an exocytotic process necessary for fertilization. In addition, several TRPCs have been detected heterogeneously distributed in mouse sperm, suggesting that they may participate in other functions such as motility. Using reverse transcription-polymerase chain reaction (RT-PCR) analysis, RNA messengers for TRPC1, 3, 6 and 7 were found in human spermatogenic cells. Confocal indirect immunofluorescence revealed the presence of TRPC1, 3, 4 and 6 differentially localized in the human sperm, and immunogold transmission electron microscopy indicated that TRPC epitopes are mostly associated to the surface of the cells. Because all of them were detected in the flagellum, TRPC channel antagonists were tested in sperm motility using a computer-assisted assay. Our results provide what is to our knowledge the first evidence that these channels may influence human sperm motility. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.