Salivary apyrases of Triatoma infestans are assembled into homo-oligomers
Salivary apyrases of Triatoma infestans are assembled into homo-oligomers
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DOI：
10.1042/bj20052019
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发表时间：
2006-06-15
影响因子：
4.1
通讯作者：
Teixeira, Antonio R. L.
中科院分区：
文献类型：
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作者：
Faudry, Eric;Santana, Jaime M.;Teixeira, Antonio R. L.
吸血节肢动物的唾液中存在腺苷三磷酸双磷酸酶活性。它与血液喂养有关，因为腺苷三磷酸双磷酸酶能够水解 ADP（血小板聚集的关键成分）。在恰加斯病媒介 Triatoma infestans 的唾液中鉴定出五种表观分子质量分别为 88、82、79、68 和 67 kDa 的腺苷三磷酸双磷酸酶。在这些酶的纯化过程中观察到的大尺寸表明寡聚化。在本研究中，我们使用凝胶过滤和分析超速离心证实唾液中存在分子量为 200 kDa 的腺苷三磷酸双磷酸酶寡聚物。电泳分析表明二硫键参与均聚。此外，还检测到二硫键和 pI 的异质性，pI 范围为 4.9 至 5.4。本研究首次深入了解可溶性腺苷三磷酸双磷酸酶的四级结构。
Apyrase activity is present in the saliva of haematophagous arthropods. It is related to blood-feeding because of the apyrase ability to hydrolyse ADP, a key component of platelet aggregation. Five apyrases with apparent molecular masses of 88, 82, 79, 68 and 67 kDa were identified in the saliva of the vector of Chagas disease, Triatoma infestans. The large size observed during purification of these enzymes suggested oligomerization. In the present study, we confirmed, using gel-filtration and analytical ultracentrifugation, the presence of apyrase oligomers with molecular masses of 200 kDa in the saliva. Electrophoretic analyses showed that disulphide bonds were involved in homo-oligomerization. In addition, heterogeneity in disulphide bonds and in pI was detected, with the pI ranging from 4.9 to 5.4. The present study gives the first insights into the quaternary structure of soluble apyrases.