The significance of M1/M2 macrophage-like monocytes in children with systemic lupus erythematosus
The significance of M1/M2 macrophage-like monocytes in children with systemic lupus erythematosus
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DOI：
10.1177/2058739218824463
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发表时间：
2019-01
影响因子：
0.7
通讯作者：
X. Niu;D. Feng;S. Hao;X. Kuang;Ying Wu;G. Zhu;Wenyan Huang
中科院分区：
文献类型：
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作者：
X. Niu;D. Feng;S. Hao;X. Kuang;Ying Wu;G. Zhu;Wenyan Huang
单核细胞/巨噬细胞在系统性红斑狼疮的发展中是重要的。为研究系统性红斑狼疮（SLE）患儿外周血中M1、M2类巨噬细胞的表达，探讨其临床意义，采用流式细胞术检测SLE患儿外周血中M1、M2类巨噬细胞、肿瘤坏死因子-α、白细胞介素-1、白细胞介素-6、白细胞介素-10、白细胞介素-18。对M1、M2型巨噬细胞样单核细胞与血沉、C反应蛋白进行相关分析。结果发现，重症SLE患者外周血M1型巨噬细胞样单核细胞（CD 163-CD 14+）的绝对数量和占巨噬细胞样单核细胞（CD 14+）的百分比均高于对照组和轻、中度SLE患者（F = 28.4，21.7，122，81.7; P < 0.05）。但M2巨噬细胞样单核细胞（CD 163 + CD 14+）的绝对数量在三组间无明显差异。M1型巨噬细胞样单核细胞的绝对数量和占巨噬细胞样单核细胞的百分比与C反应蛋白和血沉呈正相关（r = 0.46，0.44，0.367，0.47; P < 0.05）;然而，巨噬细胞中M2巨噬细胞样单核细胞的绝对数量和百分比，类单核细胞与CRP、血沉呈负相关（r =-0.47、-0.45、-0.47、-0.32; P < 0.05）。因此，M1巨噬细胞样单核细胞对系统性红斑狼疮患儿的炎症有有效的影响。M2巨噬细胞样单核细胞在很大程度上具有与M1巨噬细胞样单核细胞相反的功能。在儿童系统性红斑狼疮中，巨噬细胞在系统性红斑狼疮的发生发展中起重要作用，M1巨噬细胞样单核细胞在活动期系统性红斑狼疮中起重要作用，可诱导炎症反应，并与系统性红斑狼疮的严重程度相关。
Monocytes/macrophages are important in the development of systemic lupus erythematosus. To research M1 and M2 macrophage-like monocytes in the peripheral blood of children with systemic lupus erythematosus and explore the clinical significance, M1 and M2 macrophage-like monocytes, tumor necrosis factor-α, interleukin-1, interleukin-6, interleukin-10, and interleukin-18 are tested in the peripheral blood of children with systemic lupus erythematosus by flow cytometry. A correlation analysis is made between M1 and M2 macrophage-like monocytes and erythrocyte sedimentation rate and C-reactive protein. As we found, the absolute number and percentage of M1 macrophage-like monocytes (CD163–CD14+) in macrophage-like monocytes (CD14+) in peripheral blood of the severe systemic lupus erythematosus group were higher than those of the control group and the mild–moderate systemic lupus erythematosus group (F = 28.4, 21.7, 122, 81.7; P < 0.05). But there was no obvious difference between these three groups in terms of the absolute number of M2 macrophage-like monocytes (CD163+CD14+). The absolute number and percentage of M1 macrophage-like monocytes in macrophage-like monocytes had positive correlation with C-reactive protein and erythrocyte sedimentation rate (r = 0.46, 0.44, 0.367, 0.47; P < 0.05); whereas, the absolute number and percentage of M2 macrophage-like monocytes in macrophage-like monocytes had negative correlation with CRP and erythrocyte sedimentation rate (r = –0.47, –0.45, –0.47, –0.32; P < 0.05). Thus, M1 macrophage-like monocytes have effective impact on inflammation in children with systemic lupus erythematosus. M2 macrophage-like monocytes, to a large extent, have the opposite functions compared to M1 macrophage-like monocytes. In children with systemic lupus erythematosus, macrophages play important role in the development of systemic lupus erythematosus and M1 macrophage-like monocytes have functions in active systemic lupus erythematosus and they can induce the inflammation and have correlation with severity of systemic lupus erythematosus.