ADRENAL INNERVATION MAY BE AN EXTRAPITUITARY MECHANISM ABLE TO REGULATE ADRENOCORTICAL RHYTHMICITY IN RATS
ADRENAL INNERVATION MAY BE AN EXTRAPITUITARY MECHANISM ABLE TO REGULATE ADRENOCORTICAL RHYTHMICITY IN RATS
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DOI：
10.1210/endo-111-4-1334
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发表时间：
1982-01-01
期刊：
影响因子：
4.8
通讯作者：
MEIER, AH
中科院分区：
文献类型：
--
作者：
OTTENWELLER, JE;MEIER, AH
在未切除垂体的大鼠和植入含有 ACTH 和 T4 [甲状腺素]的蜂蜡颗粒的垂体切除大鼠中研究了血浆皮质类固醇浓度的每日节律。在第一个实验中，将垂体切除的雄性大鼠置于12小时光照、12小时黑暗周期（光照开始，0600小时），并随意提供食物和水。皮下植入含有1.5mg ACTH和150μg T4的蜂蜡颗粒。在这些老鼠身上。从植入ACTH和T4后4天开始，连续3天检测这些大鼠血浆皮质类固醇浓度的日节律。腹腔注射戊巴比妥钠（40mg/kg体重）或硫酸阿托品（10mg/kg体重）在这些大鼠和一组完整大鼠中，1200 小时阻断了 1800 小时血浆皮质类固醇浓度的预期上升。在第二个实验中，T-7 水平的脊髓横断破坏了肾上腺神经支配。 L-1横断大鼠作为手术对照，第三组作为未手术对照。手术后一周，未手术对照组和 L-1 对照组均存在血浆皮质类固醇浓度的日节律（P < 0.01），但 T-7 横断大鼠中则没有。由于垂体外机制能够维持垂体切除大鼠的肾上腺皮质节律性，并且肾上腺神经支配的破坏抑制了肾上腺皮质节律性，因此假设肾上腺神经支配可能是在肾上腺皮质节律性中起作用的垂体外机制。
The daily rhythm of plasma corticosteroid concentration was studied in nonhypophysectomized rats and in hypophysectomized rats implanted with beeswax pellets containing ACTH and T4 [thyroxine]. In the 1st experiment, male hypophysectomized rats were placed on a 12 h light, 12 h dark cycle (onset of light, 0600 h) with food and water available ad libitum. Beeswax pellets containing 1.5 mg ACTH and 150 .mu.g T4 were implanted s.c. in these rats. Beginning 4 days after the implantation of ACTH and T4, daily rhythms of plasma corticosteroid concentration were detected in these rats on 3 successive days. The injection of sodium pentobarbital (40 mg/kg body wt) or atropine sulfate (10 mg/kg body wt) i.p. at 1200 h blocked the expected rise in plasma corticosteroid concentration at 1800 h in both these rats and a group of intact rats. In the 2nd experiment, adrenal innervation was disrupted by spinal cord transection at the T-7 level. L-1-transected rats served as operated controls, and a 3rd group was maintained as unoperated controls. One week after surgery, daily rhythms of plasma corticosteroid concentration were present in both unoperated and L-1 controls (P < 0.01) but not in the T-7-transected rats. Inasmuch as an extrapituitary mechanism was capable of maintaining adrenocortical rhythmicity in hypophysectomized rats and disruption of adrenal innervation suppressed adrenocortical rhythmicity, it was hypothesized that adrenal innervation may be an extrapituitary mechanism which has a role in adrenocortical rhythmicity.