Biodegradation of pentachloronitrobenzene by Cupriavidus sp. YNS-85 and its potential for remediation of contaminated soils
Biodegradation of pentachloronitrobenzene by Cupriavidus sp. YNS-85 and its potential for remediation of contaminated soils
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DOI：
10.1007/s11356-017-8640-2
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发表时间：
2017-02
影响因子：
5.8
通讯作者：
Ying Teng;Xiaomi Wang;Ye Zhu;Wei Chen-;P. Christie;Zhen-gao Li;Yongming Luo
中科院分区：
文献类型：
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作者：
Ying Teng;Xiaomi Wang;Ye Zhu;Wei Chen-;P. Christie;Zhen-gao Li;Yongming Luo
五氯硝基苯（PCNB）是一种有毒的氯化硝基芳香族化合物。然而，据报道，只有少数细菌能够利用PCNB。在本研究中，一种五氯硝基苯（PCNB）降解细菌Cupriavidussp。nns -85是从一株污染三七中分离得到的。菌株在水溶液中共代谢200 mg L−1PCNB， 5天后去除率为73.8%。该细菌在酸性条件下（pH值4-6）也能有效降解PCNB，并表现出对有毒微量元素（砷、铜和镉）的抗性。它利用PCNB中间体作为唯一碳源的能力也得到了证实。土壤微观环境实验进一步表明，细菌生物强化处理可提高土壤中PCNB的去除率（37.8%）和五氯苯胺的积累量（89.3%）。生物修复对土壤酶活性和微生物群落功能多样性有积极影响。这些发现表明，cupriavidussp。YNS-85可能是一种适合于pcnb污染场地的原位生物修复的接种剂，特别是对重金属（样物质）共污染的酸性土壤。
Pentachloronitrobenzene (PCNB) is a toxic chlorinated nitroaromatic compound. However, only a few bacteria have been reported to be able to utilize PCNB. In the present study, one pentachloronitrobenzene (PCNB)-degrading bacterium,Cupriavidussp. YNS-85, was isolated from a contaminatedPanax notoginsengplantation. The strain co-metabolized 200 mg L−1PCNB in aqueous solution with a removal rate of 73.8% after 5 days. The bacterium also degraded PCNB effectively under acid conditions (pH 4–6) and showed resistance to toxic trace elements (arsenic, copper, and cadmium). Its ability to utilize proposed PCNB intermediates as sole carbon sources was also confirmed. The soil microcosm experiment further demonstrated that bacterial bioaugmentation enhanced the removal of PCNB (37.8%) from soil and the accumulation of pentachloroaniline (89.3%) after 30 days. Soil enzyme activity and microbial community functional diversity were positively influenced after bioremediation. These findings indicate thatCupriavidussp. YNS-85 may be a suitable inoculant for in situ bioremediation of PCNB-polluted sites, especially those with acid soils co-contaminated with heavy metal(loid)s.