Galleria mellonella as a Model System To Study Acinetobacter baumannii Pathogenesis and Therapeutics
Galleria mellonella as a Model System To Study Acinetobacter baumannii Pathogenesis and Therapeutics
复制标题
DOI：
10.1128/aac.01533-08
复制
发表时间：
2009-06-01
影响因子：
4.9
通讯作者：
Mylonakis, Eleftherios
中科院分区：
文献类型：
--
作者：
Peleg, Anton Y.;Jara, Sebastian;Mylonakis, Eleftherios
与哺乳动物模型相比，非哺乳动物感染模型系统，如mellonella Galleria（大蜡蛾的毛虫）具有显著的后勤和伦理优势。在这项研究中，我们利用大黄蜂毛虫研究宿主-病原体与革兰氏阴性菌鲍曼不动杆菌的相互作用，并确定这种感染模型在研究抗菌效果方面的效用。感染后与参考a . g . mellonella毛毛虫baumannii应变,我们观察到g . mellonella杀人的速度取决于感染培养液和孵化温度postinfection,与死亡37 C大于30度(P = 0.01)。鲍曼不动杆菌致死率高于致病性较低的贝氏不动杆菌和洛菲不动杆菌（P < 0.001）。社区获得性鲍曼不动杆菌致死率高于医院获得性菌株（P < 0.01）。群体感应分子3-羟基- c -12-高丝氨酸内酯的产生水平的降低没有引起鲍曼不动杆菌对大黄蜂的毒力的变化。用体外抗感染鲍曼不动杆菌菌株的抗生素治疗致命鲍曼不动杆菌感染，与用细菌耐药的抗生素治疗相比，可显著延长黄蜡不动杆菌毛虫的存活时间。麦香杆菌是一种相对简单的非哺乳动物模型系统，可用于促进鲍曼不动杆菌宿主-病原体相互作用和抗菌药物疗效的体内研究。
Nonmammalian model systems of infection such as Galleria mellonella (caterpillars of the greater wax moth) have significant logistical and ethical advantages over mammalian models. In this study, we utilize G. mellonella caterpillars to study host-pathogen interactions with the gram-negative organism Acinetobacter baumannii and determine the utility of this infection model to study antibacterial efficacy. After infecting G. mellonella caterpillars with a reference A. baumannii strain, we observed that the rate of G. mellonella killing was dependent on the infection inoculum and the incubation temperature postinfection, with greater killing at 37 C than at 30 C (P = 0.01). A. baumannii strains caused greater killing than the less-pathogenic species Acinetobacter baylyi and Acinetobacter lwoffii (P < 0.001). Community-acquired A. baumannii caused greater killing than a reference hospital-acquired strain (P < 0.01). Reduced levels of production of the quorum-sensing molecule 3-hydroxy-C-12-homoserine lactone caused no change in A. baumannii virulence against G. mellonella. Treatment of a lethal A. baumannii infection with antibiotics that had in vitro activity against the infecting A. baumannii strain significantly prolonged the survival of G. mellonella caterpillars compared with treatment with antibiotics to which the bacteria were resistant. G. mellonella is a relatively simple, nonmammalian model system that can be used to facilitate the in vivo study of host-pathogen interactions in A. baumannii and the efficacy of antibacterial agents.