Microchannel electrophoretic separations of DNA in injection-molded plastic substrates
Microchannel electrophoretic separations of DNA in injection-molded plastic substrates
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DOI：
10.1021/ac9701997
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发表时间：
1997-07-15
影响因子：
7.4
通讯作者：
Hooper, HH
中科院分区：
文献类型：
--
作者：
McCormick, RM;Nelson, RJ;Hooper, HH
微制造电泳分离装置已经通过注射成型工艺生产。生产这种设备的策略包括在硅晶片上对母模进行液相蚀刻，然后从硅母模上电铸出更耐用的镍注塑母模。其中一个镍电铸件用于制备注塑模具插件，从该插件批量生产丙烯酸基板中的微通道芯片。微通道装置被用来证明双链DNA片段的高分辨率分离，总运行时间小于3分钟。运行到运行和芯片到芯片的重现性良好，运行到运行数据的相对标准偏差值低于1%，芯片到芯片的比较范围为2-3%。这样的设备可以导致生产低成本，一次性使用的电泳芯片，适用于各种分离应用，包括DNA大小，DNA测序，随机初级文库筛选，和快速免疫测定测试。
Microfabricated electrophoretic separation devices have been produced by an injection-molding process. The strategy for producing the devices involved solution-phase etching of a master template on a silicon wafer, followed by electroforming more durable injection-molding masters in nickel from the silicon master. One of the nickel electroforms was than used to prepare an injection mold insert, from which microchannel chips in an acrylic substrate were mass-produced. The microchannel devices were used to demonstrate high-resolution separations of double-stranded DNA fragments with total run times of less than 3 min. Run-to-run and chip-to-chip reproductibility was good, with relative standard deviation values below 1% for the run-to-run data and in the range of 2-3% for the chip-to-chip comparisons. Such devices could lead to the production of low-cost, single-use electrophoretic chips suitable for a variety of separation applications, including DNA sizing, DNA sequencing, random primary library screening, and rapid immunoassay testing.