HUMAN IMMUNODEFICIENCY VIRUS-INFECTION OF THE BRAIN .1. VIRUS ISOLATION AND DETECTION OF HIV SPECIFIC ANTIBODIES IN THE CEREBROSPINAL-FLUID OF PATIENTS WITH VARYING CLINICAL CONDITIONS
HUMAN IMMUNODEFICIENCY VIRUS-INFECTION OF THE BRAIN .1. VIRUS ISOLATION AND DETECTION OF HIV SPECIFIC ANTIBODIES IN THE CEREBROSPINAL-FLUID OF PATIENTS WITH VARYING CLINICAL CONDITIONS
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DOI：
10.1016/0022-510x(88)90184-0
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发表时间：
1988-07-01
影响因子：
4.4
通讯作者：
FENYO, EM
中科院分区：
文献类型：
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作者：
CHIODI, F;SONNERBORG, A;FENYO, EM
从29例不同程度HIV感染患者的脑脊液（CSF）中分离出人类免疫缺陷病毒（HIV）。病毒可以从所有疾病阶段的患者中分离出来，包括原发性HIV感染患者和无症状携带者。在感染的早期，游离病毒很少出现在脑脊液中，但可以从脑脊液中存在的细胞中分离出来。这表明艾滋病毒可能在感染的非常早期阶段就到达大脑，并且最初受感染的细胞很少产生病毒。在HIV感染的晚期，与免疫缺陷的严重程度增加有关，游离病毒可以很容易地从CSF中分离出来。除了一个例外，所有这些患者都有神经和/或精神症状，而在感染的早期阶段只有2名（13名）受试者。所有血清中有hiv特异性抗体的患者在脑脊液中也有抗体。通过放射免疫沉淀测定，脑脊液中含有病毒核心蛋白前体（p55）抗体的情况比患者相应的血清中多。我们提出，免疫紊乱在与HIV感染相关的神经/精神症状中具有重要的致病作用，可能是通过允许中枢神经系统中病毒表达增加。
Isolation of the human immunodeficiency virus (HIV) has been attempted from the cerebrospinal fluid (CSF) of 29 subjects with varying severity of HIV infection. Virus could be isolated from patients in all stages of disease including patients with primary HIV infection and asymptomatic carriers. In the early stages of infection free virus was infrequently present in the CSF but could be isolated from the cells present in CSF. This suggests that HIV may reach the brain at a very early stage of infection and that initially there is little production of virus from infected cells. In the late stages of HIV infection, associated with increasing severity of immunodeficiency, free virus could readily be isolated from the CSF. With one exception, all these patients had neurological and/or psychiatric symptoms, as compared to only 2 (of 13) subjects in the early stages of infection. All patients with HIV-specific antibodies in serum had antibodies also in CSF. Examined by a radioimmunoprecipitation assay, CSF was more often found to contain antibodies to the precursor (p55) of viral core proteins than the corresponding serum of the patients. We propose that immune disturbances have an essential pathogenic role in the neurological/psychiatric symptoms associated with HIV infection, possibly through allowing increased viral expression in the central nervous system.