The Dynamin Related Protein Dnm1 Fragments Mitochondria in a Microtubule-Dependent Manner During The Fission Yeast Cell Cycle
The Dynamin Related Protein Dnm1 Fragments Mitochondria in a Microtubule-Dependent Manner During The Fission Yeast Cell Cycle
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DOI：
10.1002/cm.20351
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发表时间：
2009-08-01
影响因子：
--
通讯作者：
Hyams, Jeremy S.
中科院分区：
文献类型：
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作者：
Jourdain, Isabelle;Gachet, Yannick;Hyams, Jeremy S.
线粒体是动态的细胞器，经历裂变和融合的循环。在裂殖酵母裂殖酵母中，线粒体与微管对齐，线粒体的完整性取决于完整的微管细胞骨架。在这里，我们表明，线粒体重组在细胞周期中，这一过程是动力和微管依赖。微管解聚导致线粒体片段化，但仅当动力蛋白相关蛋白Dnm 1存在时。另一方面，线粒体融合不依赖于微管。dnm 1 Delta细胞除了显示出广泛融合的线粒体外，还对抗微管药物具有特异性耐药性。Dnm 1-YFP定位于线粒体切割位点的病灶，这些位点占据相邻类核之间的界面，表明存在定义的线粒体“领土”，每个领土都包含一个类核。这样的领土在dnm 1 Delta中丢失，其中类核聚集。线粒体末端表现出独立于细胞骨架的运动行为，朝向细胞极延伸和从细胞极缩回。我们的结论是：（a）线粒体由微管组织在分裂酵母中，但不被它们移动;（B）Dnm 1在间期生长和细胞分裂期间介导线粒体分裂;（c）微管和线粒体之间的相互作用，直接或间接地通过Dnm 1，不仅改变线粒体的处置，还改变微管的行为。细胞动力。Cytoskeleton 66：509-523，2009. (C)2009 Wiley-Liss，Inc.
Mitochondria are dynamic organelles that undergo cycles of fission and fusion. In the fission yeast, Schizosaccharomyces pombe, mitochondria align with microtubules and mitochondrial integrity is dependent upon an intact microtubule cytoskeleton. Here we show that mitochondria re-organize during the cell cycle and that this process is both dynamin- and microtubule-dependent. Microtubule depolymerization results in mitochondrial fragmentation but only when the dynamin-related protein Dnm1 is present. Mitochondrial fusion is, oil the other hand, microtubule-independent. dnm1 Delta cells, besides showing extensively fused mitochondria, are specifically resistant to anti-microtubule drugs. Dnm1-YFP localizes to foci at sites of mitochondrial severing which occupy the interface between adjacent nucleoids, suggesting the existence of defined mitochondrial "territories," each of which contains a nucleoid. Such territories are lost in dnm1 Delta in which nucleoids become aggregated. Mitochondrial ends exhibit motile behavior, extending towards and retracting from the cell poles, independently of the cytoskeleton. We conclude that: (a) mitochondria are organized by microtubules ill fission yeast but are not moved by them; (b) Dnm1 mediates mitochondrial fission during interphasic growth and at cell division; (c) the interaction between microtubules and mitochondria, either directly or indirectly via Dnm1, not only modifies the disposition of mitochondria it also modifies the behavior of microtubules. Cell Motil. Cytoskeleton 66: 509-523, 2009. (C) 2009 Wiley-Liss, Inc.