Quantitative gingival crevicular fluid proteome in health and periodontal disease using stable isotope chemistries and mass spectrometry.
Quantitative gingival crevicular fluid proteome in health and periodontal disease using stable isotope chemistries and mass spectrometry.
复制标题
DOI：
10.1111/jcpe.12262
复制
发表时间：
2014-08
影响因子：
6.7
通讯作者：
Salih E
中科院分区：
文献类型：
--
作者：
Carneiro LG;Nouh H;Salih E
应用定量稳定同位素标记化学和质谱（MS）确定牙周病龈沟液（GCF）蛋白质组的变化。使用320个GCF样本和稳定同位素标记试剂ICAT和mTRAQ，利用MS技术建立了40名健康人和40名牙周病患者GCF的定量蛋白质组，并通过酶联免疫吸附法进行了验证。我们已经确定了238种不同的蛋白质，其中180种在健康和牙周患者的GCF中进行了定量，另外26种和32种不同的蛋白质仅在健康或牙周患者的GCF中发现。此外，还对42种致病细菌蛋白和11种酵母蛋白进行了定量。这些数据突出显示了一系列先前未通过大规模MS方法在GCF中定量的与牙周病相关的蛋白质，例如宿主来源的IG α-2链C、激肽释放酶-4、S100-A9、跨膜蛋白酶13、肽酶S1结构域、几种胶原类型和致病性细菌蛋白，甲酰胺酶、亮氨酸酰胺肽酶和毒力因子OMP 85。创新的分析方法提供了详细的新的变化，在宿主和微生物来源的牙周病患者的GCF蛋白质组。该研究确定了50种宿主蛋白和16种致病细菌蛋白在牙周病中显著升高，其中大部分是新的，在牙周病的临床竞技场中具有重要的应用潜力。
Application of quantitative stable-isotope-labeling chemistries and mass spectrometry (MS) to determine alterations in gingival crevicular fluid (GCF) proteome in periodontal disease. Quantitative proteome of GCF from 40 healthy individuals versus 40 patients with periodontal disease was established using 320 GCF samples and stable-isotope-labeling reagents, ICAT and mTRAQ, with MS technology and validated by enzyme-linked immunosorbent methods. We have identified 238 distinct proteins of which 180 were quantified in GCF of both healthy and periodontal patients with additional 26 and 32 distinct proteins that were found only in GCF of healthy or periodontal patients. In addition, 42 pathogenic bacterial proteins and 11 yeast proteins were quantified. The data highlighted a series of proteins not quantified previously by large-scale MS approaches in GCF with relevance to periodontal disease, such as host derived Ig alpha-2 chain C, Kallikrein-4, S100-A9, transmembrane proteinase 13, peptidase S1 domain, several collagen types and pathogenic bacterial proteins e.g., formamidase, leucine amidopeptidase and virulence factor OMP85. The innovative analytical approaches provided detailed novel changes in both host and microbial derived GCF proteomes of periodontal patients. The study defined 50 host and 16 pathogenic bacterial proteins significantly elevated in periodontal disease most of which were novel with significant potential for application in the clinical arena of periodontal disease.
登录
查看更多内容
影响因子：
7.6
作者：
Kojima, T;Andersen, E;Cimasoni, G
通讯作者：
Cimasoni, G
影响因子：
3.4
作者：
Hu, S;Xie, YM;Wong, DT
通讯作者：
Wong, DT
影响因子：
4.4
作者：
Bealer, John F.;Colgin, Mark
通讯作者：
Colgin, Mark
影响因子：
4.4
作者：
Bostanci, Nagihan;Ramberg, Per;Papapanou, Panos N.
通讯作者：
Papapanou, Panos N.
影响因子：
18.6
作者：
Champagne, CME;Buchanan, W;Offenbacher, S
通讯作者：
Offenbacher, S