Neuropilin-1 Interacts with Fibronectin-1 to Promote Epithelial-Mesenchymal Transition Progress in Gastric Cancer.
Neuropilin-1 Interacts with Fibronectin-1 to Promote Epithelial-Mesenchymal Transition Progress in Gastric Cancer.
复制标题
Neuropilin-1 与 Fibronectin-1 相互作用促进胃癌上皮-间质转化进展
DOI：
10.2147/ott.s275327
复制
发表时间：
2020
影响因子：
4
通讯作者：
Li H
中科院分区：
文献类型：
--
作者：
Wu C;Zeng MH;Liao G;Qian K;Li H
神经纤毛蛋白-1（NRP 1）与许多配体和共受体结合，影响细胞存活和迁移，这对肿瘤进展至关重要。然而，关于NRP 1如何影响上皮-间质转化（EMT）相关的胃癌恶性进展仍有很大的未知数。我们使用TCGA分析胃癌中NRP 1的表达及其对患者生存的影响。体外实验采用transwell法、伤口愈合法和集落形成法研究NRP 1和NRP 3对胃癌细胞侵袭、迁移和增殖的影响。在体内实验中，我们评估了NRP 1的过表达和敲低以及NRP 3对肿瘤生长的影响。我们发现NRP 1在进展期胃癌中高表达，与预后不良相关。NRP 1基因表达下调可抑制胃癌细胞的增殖和转移。机械地。NRP 1与FN 1相互作用，通过ECM重塑促进胃癌细胞的恶性进展。此外，我们发现，NRP 3可以阻断NRP 1和FN 1的相互作用，抑制胃癌的进展。我们的研究表明，NRP 1和FN 1的相互作用是至关重要的胃癌的恶性进展。这可能为胃癌的治疗提供新的视角和潜在的治疗方法。
Neuropilin-1 (NRP1) binds to many ligands and co-receptors and affects cell survival and migration, which is essential for tumor progression. However, there are still largely unknowns about how NRP1 affects the epithelial–mesenchymal transition (EMT)-related malignant progression in gastric cancer. We used TCGA to analyze the expression of NRP1 in gastric cancer and its impact on patient survival. In in vitro experiments, transwell, wound healing and colony formation assays were used to evaluate the effects of NRP1 and ginsenoside Rg3 on the invasion, migration and proliferation of gastric cancer cells. In in vivo experiments, we evaluated the overexpression and knockdown of NRP1 and the effect of ginsenoside Rg3 on tumor growth. We found that NRP1 is highly expressed in advanced gastric cancer and associated with poor prognosis. Knockdown of NRP1 expression can inhibit the proliferation and metastasis of gastric cancer cells. Mechanically. NRP1 interacts with fibronectin-1 (FN1) to promote the malignant progression of gastric cancer cells through ECM remodeling. In addition, we found that ginsenoside Rg3 can block the interaction of NRP1 and FN1 and inhibit the progression of gastric cancer. Our study suggested that the interaction of NRP1 and FN1 is crucial for the malignant progression of gastric cancer. This may provide a new perspective and potential treatment methods for the treatment of gastric cancer.