Serologic and mucosal immune response to rotavirus infection in the rabbit model.
Serologic and mucosal immune response to rotavirus infection in the rabbit model.
复制标题
兔模型中轮状病毒感染的血清学和粘膜免疫反应。
DOI：
10.1128/jvi.65.5.2562-2571.1991
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发表时间：
1991
影响因子：
5.4
通讯作者：
Graham,DY
中科院分区：
文献类型：
--
作者：
Conner,ME;Gilger,MA;Estes,MK;Graham,DY
我们检测了特定无病原体家兔接种和口服兔Ala轮状病毒(7.5 × 10（5)至1 × 10(7) PFU）后对轮状病毒感染的体液免疫反应。采用总抗体酶联免疫吸附法（elisa）、同种型特异性elisa和斑块减少中和法监测血清学和粘膜样品的体液免疫反应。初次感染后，在接种后1周检测所有家兔的脱落病毒、血清学和粘膜抗体反应。接种3天后检测肠道免疫球蛋白M，接种6天后检测分泌性免疫球蛋白A。攻毒后，兔子被保护（没有检测到病毒脱落）免受感染。仅在粘膜中和抗体中观察到遗忘性免疫反应，并且所有血清学和粘膜免疫反应都保持在高水平，至少持续到攻毒后175天（接种后204天）。中和反应的检测受中和试验所用病毒株的影响；所有接种的家兔均产生可检测到的抗感染（Ala）病毒株的血清和肠道中和抗体。初次和攻毒接种Ala病毒后，血清和粘膜样品中的中和活性普遍呈同型（VP7血清型3），但并非完全相同。在血清8型（12只兔中有9只）和9型（12只兔中有12只）病毒中观察到异型血清中和活性，可能是基于与外衣壳蛋白VP4或VP7 C区共享表位的反应性。粘膜和血清学样本中异种（血清型3）和异种中和反应的比较显示43%（49例中的21例）的反应不一致。49例患者中有19例（39%）在没有异型黏膜反应的情况下出现异型血清学反应，但49例患者中有2例（4%）在没有伴随血清学反应的情况下出现异型黏膜反应。这些结果提供了深入了解可能影响检测异型反应的因素。
We examined the humoral immune response to rotavirus infection in specific pathogen-free rabbits inoculated and challenged orally with rabbit Ala rotavirus (7.5 x 10(5) to 1 x 10(7) PFU). The humoral immune response in both serologic and mucosal samples was monitored by using total antibody enzyme-linked immunosorbent assays (ELISAs), isotype-specific ELISAs, and plaque reduction neutralization assays. Following a primary infection, all rabbits shed virus and serologic and mucosal antibody responses were initially detected by 1 week postinoculation. Intestinal immunoglobulin M was detected by 3 days postinoculation, and secretory immunoglobulin A was detected by 6 days postinoculation. Following challenge, rabbits were protected (no detectable virus shedding) from infection. An anamnestic immune response was observed only with mucosal neutralizing antibodies, and all serologic and mucosal immune responses persisted at high levels until at least 175 days postchallenge (204 days postinoculation). Detection of neutralization responses was influenced by the virus strain used in the neutralization assay; all inoculated rabbits developed detectable serum and intestinal neutralizing antibodies against the infecting (Ala) virus strain. Neutralization activity in both serum and mucosal samples was generally, but not exclusively, homotypic (VP7 serotype 3) after both primary and challenge inoculations with Ala virus. Heterotypic serum neutralization activity was observed with serotype 8 (9 of 12 rabbits) and 9 (12 of 12 rabbits) viruses and may be based on reactivity with the outer capsid protein VP4 or on a shared epitope in the C region of VP7. Comparisons of heterologous (serotype 3) and heterotypic neutralizing responses in mucosal and serologic samples revealed that 43% (21 of 49) of the responses were discordant. In 19 of 49 (39%) of these cases, a heterotypic serologic response was seen in the absence of a heterotypic mucosal response, but in 2 of 49 (4%) instances, a heterotypic mucosal response was seen in the absence of a concomitant serologic response. These results provide insight into factors which may affect detection of heterotypic responses.