Antisense Long Noncoding RNA HIF1A-AS2 Is Upregulated in Gastric Cancer and Associated with Poor Prognosis
Antisense Long Noncoding RNA HIF1A-AS2 Is Upregulated in Gastric Cancer and Associated with Poor Prognosis
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DOI：
10.1007/s10620-015-3524-0
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发表时间：
2015-06-01
影响因子：
3.1
通讯作者：
Shu, Yong-qian
中科院分区：
文献类型：
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作者：
Chen, Wen-ming;Huang, Ming-de;Shu, Yong-qian
背景长链非编码RNA（lncRNA）最近被证明在基本生物学过程中发挥重要的调节作用，其中许多在多种人类癌症中被失调。缺氧诱导因子1 α反义RNA-2 HIF 1A-AS 2在非乳头状透明细胞肾癌中过度表达，并参与肿瘤的发生、发展。目的探讨HIF 1A-AS 2在胃癌中的表达及其生物学功能。材料与方法采用实时荧光定量聚合酶链反应（Real-time PCR）检测HIF 1A-AS 2在胃癌中的表达水平。胃癌组织中的AS 2。分析其表达与临床病理特征的相关性。构建受试者工作特征曲线下面积（ROCAUC）评价HIF 1A-AS 2的诊断价值。结果HIF 1A-AS 2在胃癌组织中的表达明显高于癌旁正常组织（P <0.001）; HIF 1A-AS 2在胃癌组织中的表达明显高于癌旁正常组织（P < 0.001）;其过表达与肿瘤的TNM分期（P = 0.008）、浸润深度（P = 0.016）、淋巴结转移（P = 0.042）及预后不良（P = 0.001）有关。HIF 1A-AS 2的ROCAUC为0.673（95%CI 0.596-0.744，P < 0.001）。结论HIF 1A-AS 2在胃癌中过表达，可能在肿瘤细胞增殖中起重要作用。它可以作为一个潜在的诊断和预后的生物标志物GC。
Background Long noncoding RNAs (lncRNAs) have been recently shown to play important regulatory roles in fundamental biological processes, and many of them are deregulated in several human cancers. LncRNA hypoxia-inducible factor 1alpha antisense RNA-2 (HIF1A-AS2) is overexpressed in nonpapillary clear-cell renal carcinomas and involved in cancer progression.Aim This study was to evaluate the expression of HIF1A-AS2 in gastric cancer (GC) and further explore its biological function in GC cells.Materials and Methods Quantitative real-time polymerase chain reaction was used to detect the expression level of HIF1A-AS2 in GC tissues. The correlation of its expression with clinicopathological features was analyzed. Area under receiver operating characteristic curve (ROCAUC) was constructed to evaluate the diagnostic value of HIF1A-AS2. Besides, tumor cell proliferation was assessed following knockdown of HIF1A-AS2, by MTT and colony formation assay in vitro, and tumor formation assay in a nude mouse model in vivo.Results The expression of HIF1A-AS2 was upregulated in GC tumorous tissues compared with the adjacent normal tissues (P < 0.001). Its overexpression was correlated with TNM stages (P = 0.008), tumor invasion (P = 0.016), lymph node metastasis (P = 0.042), and poor prognosis (P = 0.001). In addition, ROCAUC of HIF1A-AS2 was up to 0.673 (95 % CI 0.596-0.744, P < 0.001). Moreover, knockdown of HIF1A-AS2 expression by siRNA could inhibit cell proliferation in vitro and tumorigenesis in vivo.Conclusions HIF1A-AS2 is overexpressed in GC and may play a pivotal role in tumor cell proliferation. It can be used as a potential diagnostic and prognostic biomarker for GC.