Sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for (anti‐human T‐cell leukemia virus type I) IgG in serum using a synthetic peptide, Cys‐Env gp46(188‐224), as antigen
Sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for (anti‐human T‐cell leukemia virus type I) IgG in serum using a synthetic peptide, Cys‐Env gp46(188‐224), as antigen
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使用合成肽 Cys-Env gp46(188-224) 作为抗原，对血清中的（抗人 T 细胞白血病病毒 I 型）IgG 进行灵敏酶免疫分析（免疫复合物转移酶免疫分析）
DOI：
10.1002/jcla.1860060206
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发表时间：
1992
影响因子：
2.7
通讯作者：
E. Ishikawa
中科院分区：
文献类型：
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作者：
T. Kohno;I. Sakoda;E. Ishikawa
描述了使用HTLV-I的合成肽Cys-env gp 46（188 - 224）测定血清中（抗人T细胞白血病病毒I型）IgG（抗HTLV-I IgG）的灵敏酶免疫测定法（免疫复合物转移酶免疫测定法）。将试验血清中的抗HTLV-I IgG与过量的无活性β-D-半乳糖苷酶孵育以消除抗β-D-半乳糖苷酶抗体的干扰，同时与2，4-二硝基苯基-牛血清白蛋白-Cys-env gp 46（188 - 224）结合物和Cys-env gp 46（188 - 224）-β-D-半乳糖苷酶结合物反应。将由三种组分组成的复合物捕获到涂有亲和纯化（抗2，4-二硝基苯基）IgG的聚苯乙烯球上。洗涤以消除测试血清中的非特异性IgG和过量的β-D-半乳糖苷酶缀合物后，用N-N-2，4-二硝基苯基-L-赖氨酸从聚苯乙烯球洗脱复合物，并转移到用亲和纯化的（抗人IgG-γ-链）IgG包被的聚苯乙烯球上。通过荧光测定法测定与（抗人IgG-γ-链）IgG包被的聚苯乙烯球结合的β-D-半乳糖苷酶活性。该测定法比使用Cys‐Arg‐env gp 46（188‐209）的免疫复合物转移酶免疫测定法和使用HTLV‐I作为抗原的其他方法更灵敏和有用。
A sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for (anti‐human T‐cell leukemia virus type I) IgG (anti‐HTLV‐I IgG) in serum using a synthetic peptide, Cys‐env gp46(188‐224) of HTLV‐I, is described. Anti‐HTLV‐I IgG in test serum, which had been incubated with excess of inactive β‐D‐galactosidase to eliminate interference by anti‐β‐D‐galactosidase antibodies, was reacted simultaneously with 2,4‐dinitrophenyl‐bovine serum albumin‐Cys‐env gp46 (188‐224) conjugate and Cys‐env gp46 (188‐224)‐β‐D‐galactosidase conjugate. The complex formed consisting of the three components was trapped onto polystyrene balls coated with affinity‐purified (anti‐2,4‐dinitrophenyl group) IgG. After washing to eliminate nonspecific IgG in the test serum and excess of the β‐D‐galactosidase conjugate, the complex was eluted from the polystyrene balls with ϵN‐2,4‐dinitrophenyl‐L‐lysine and transferred to polystyrene balls coated with affinity‐purified (anti‐human IgG‐γ‐chain) IgG. β‐D‐Galactosidase activity bound to the (anti‐human IgG‐γ‐chain) IgG‐coated polystyrene balls was assayed by fluorometry. This assay was more sensitive and useful than the immune complex transfer enzyme immunoassay using Cys‐Arg‐env gp46(188‐209) and other methods using HTLV‐I as antigen.
使用 env 编码的合成肽和 gp46 单克隆抗体绘制人 T 细胞白血病病毒 I 型 (HTLV-I) gp46 和 gp21 包膜糖蛋白的免疫原性区域。
DOI：
--
发表时间：
1989
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Palker,TJ;Tanner,ME;Scearce,RM;Streilein,RD;Clark,ME;Haynes,BF
通讯作者：
Haynes,BF