Radiation killing of E. coli K1060: role of membrane fluidity, hypothermia and local anaesthetics.
Radiation killing of E. coli K1060: role of membrane fluidity, hypothermia and local anaesthetics.
复制标题
大肠杆菌 K1060 的辐射杀灭：膜流动性、低温和局部麻醉剂的作用。
DOI：
10.1080/09553008014550641
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
W. Dennis
中科院分区：
文献类型：
--
作者：
M. Yatvin;B. J. Schmitz;W. Dennis
γ射线照射对大肠杆菌的杀伤作用增强。coliK1060细胞膜脂的转变温度以下，用盐酸普鲁卡因封闭。这些数据与假设一致，即与0 ℃下照射相关的杀伤增加是膜微粘度增加的结果，因为普鲁卡因已知会破坏膜。冷却增强比（c.e.r.）定义为22 ℃时的辐射D0与0 ℃时的辐射值之比。c.e.r.对于氧气鼓泡电池为1.5，对于氮气鼓泡电池为2.1。在25 mM普鲁卡因存在下，相应的c.e.r.值为1.08和1.29。氧增强比（o.e.r.）在22 ℃时为3.43，在0 ℃时为2.45。普鲁卡因的加入不会改变O.E.R.因此，温度对o.e.r.似乎与膜流动性无关。
The enhancement of killing by gamma-irradiation, which is seen when E. coli K1060 are cooled below the transition temperature of their membrane lipids, is blocked by procaine-HCl. These data are consistent with the hypothesis that increased killing associated with irradiation at 0 degree C is the result of membrane microviscosity increases, since procaine is known to fluidize membranes. A cooling enhancement ratio (c.e.r.) is defined as the ratio of radiation D0 at 22 degrees C to its value at 0 degree C. The c.e.r. for oxygen-bubbled cells is 1.5 and for nitrogen-bubbled cells is 2.1. In the presence of 25 mM procaine the respective c.e.r. values are 1.08 and 1.29. The oxygen enhancement ratio (o.e.r.) at 22 degree C is 3.43 and at 0 degree C is 2.45. The addition of procaine does not change the o.e.r. Thus, the temperature effect on o.e.r. does not appear to be related to membrane fluidity.