Use of restriction fragment length polymorphism to distinguish between salmon species.
Use of restriction fragment length polymorphism to distinguish between salmon species.
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使用限制性片段长度多态性来区分鲑鱼种类。
DOI：
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发表时间：
2000
影响因子：
6.1
通讯作者：
C. Rosa
中科院分区：
文献类型：
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作者：
V. J. Russell;G. Hold;S. Pryde;H. Rehbein;J. Quinteiro;M. Rey;C. G. Sotelo;R. Pérez;A. T. Santos;C. Rosa
研究人员使用基于 DNA 的方法对 10 种鲑鱼进行了鉴定。使用扩增线粒体细胞色素b基因区域的引物组进行DNA扩增。使用来自鲑鱼物种的 PCR 扩增 DNA 序列来选择六种限制性内切酶，从而对物种进行唯一分类。使用聚丙烯酰胺凝胶电泳解析每种酶分析后生成的 RFLP 模式，并通过银染进行可视化。结果表明，可以区分所有 10 种鲑鱼品种，并且食品行业可以轻松采用该技术来分析加工鲑鱼产品。
Identification of 10 salmon species using DNA-based methodology was investigated. Amplification of DNA was carried out using a primer set which amplified a region of the mitochondrial cytochrome b gene. Sequences of PCR-amplified DNA from the salmon species were used to select six restriction enzymes allowing species to be uniquely classified. RFLP patterns generated following analysis with each enzyme were resolved using polyacrylamide gel electrophoresis and visualized by silver staining. Results indicate that it is possible to differentiate between all 10 salmon species and that the technique could be easily adopted by the food industry for analysis of processed salmon products.