Spatial distribution of two-photon-excited fluorescence in scattering media
Spatial distribution of two-photon-excited fluorescence in scattering media
复制标题
DOI：
10.1364/ao.38.000224
复制
发表时间：
1999-01-01
期刊：
影响因子：
1.9
通讯作者：
Alfano, RR
中科院分区：
文献类型：
--
作者：
Ying, YP;Liu, F;Alfano, RR
研究了1064 nm皮秒激光脉冲作用下高散射介质表面下染料分子的双光子激发荧光（TPF）的空间分布。活性散射介质由聚苯乙烯颗粒在罗丹明590四氟硼酸盐染料溶液中的悬浮液组成。随着介质散射强度的增加，TPF强度最大值的位置逐渐向远离焦区的表面移动，TPF强度不再像非散射介质那样局限于焦区，而是分布更加均匀。用于探测散射介质的非线性光学显微镜的空间分辨率会下降。考虑到介质的散射，定性地解释了观察到的TPF空间分布。(C)1999年美国光学学会。
Spatial distribution of two-photon-excited fluorescence (TPF) for dye beneath the surface of a highly scattering medium was investigated dth picosecond laser pulses at 1064 nm. The active scattering media consisted of a suspension of polystyrene particles in a solution of Rhodamine 590 Tetrafluoroborate dye. With the increase of scattering strength of the medium, the location of the maximum TPF intensity was found to move closer to the surface away from the focal region; the intensity of TPF was not confined to the focal region as in the case of nonscattering medium but was more evenly distributed. The spatial resolution of nonlinear optical microscopy for probing a scattering medium is degraded. Taking into account the scattering of the medium qualitatively explains the observed TPF spatial distribution. (C) 1999 Optical Society of America.