Differential expression of neuropsin and protease M/neurosin in oligodendrocytes after injury to the spinal cord
Differential expression of neuropsin and protease M/neurosin in oligodendrocytes after injury to the spinal cord
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DOI：
10.1002/glia.20058
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发表时间：
2004-11-01
期刊：
影响因子：
6.2
通讯作者：
Yoshida, S
中科院分区：
文献类型：
--
作者：
Terayama, R;Bando, Y;Yoshida, S
神经蛋白酶和蛋白酶 M/神经蛋白酶是神经元和神经胶质细胞表达的丝氨酸蛋白酶，在中枢神经系统 (CNS) 中发挥多种功能。目前的研究表明，在小鼠脊髓半切后，这些蛋白酶的表达发生了变化。在未病变的脊髓中，偶尔在灰质而非白质中观察到神经蛋白酶 mRNA 表达，而白质中蛋白酶 M/神经蛋白酶 mRNA 的水平较高。脊髓损伤后，紧邻病变区域的白质中会诱导神经蛋白酶 mRNA 表达，在损伤后 4 天达到峰值，并在 14 天时消失。在病变周围的白质和灰质中观察到蛋白酶 M/神经蛋白 mRNA 的表达增强，在 4 天时达到峰值，并持续 14 天。 Neuropsin mRNA 主要由 CNPase 阳性少突胶质细胞表达。此外，大多数这些细胞还与蛋白酶 M/神经罗素蛋白的免疫反应性相关。在未损伤的脊髓内，大多数表达蛋白酶 M/神经蛋白 mRNA 的细胞是 CNPase 阳性少突胶质细胞，并且这些细胞的很大一部分还表现出 NG2（少突胶质细胞祖细胞标记物）的免疫反应性。损伤后，NG2 阳性细胞内的蛋白酶 M/神经元 mRNA 表达显着降低，而 CNPase 阳性少突胶质细胞内的组成型表达似乎得以保留。这些发现表明，基于神经蛋白酶和蛋白酶 M/神经罗素表达的每个少突胶质细胞亚群在脊髓对损伤的反应以及正常体内平衡中具有不同的作用。 (C) 2004 Wiley-Liss, Inc.
Neuropsin and protease M/neurosin are serine proteases expressed by neurons and glial cells, and serve a variety of functions in the central nervous system (CNS). The current study demonstrates changes in the expression of these proteases following hemisection of the mouse spinal cord. Within unlesioned spinal cord, neuropsin mRNA expression was occasionally observed in the gray but not white matter, while the level of protease M/neurosin mRNA was higher in the white matter. After injury to the spinal cord, neuropsin mRNA expression was induced in the white matter in the area immediately adjacent to the lesion, peaking at 4 days post-injury and disappearing by 14 days. Enhanced expression of protease M/neurosin mRNA was observed throughout the white and gray matter surrounding the lesion, peaking at 4 days and persisting for 14 days. Neuropsin mRNA was expressed predominantly by CNPase-positive oligodendrocytes. Furthermore, most of these cells were also associated with immunoreactivity for protease M/neurosin protein. Within unlesioned spinal cord, most protease M/neurosin mRNA-expressing cells were CNPase-positive oligodendrocytes, and a substantial fraction of these cells also showed immunoreactivity for NG2, a marker for oligodendrocyte progenitors. After injury, protease M/neurosin mRNA expression within NG2-positive cells was significantly decreased, while the constitutive expression in CNPase-positive oligodendrocytes appeared to be preserved. These findings suggest that each subpopulation of oligodendrocytes based on the expression of neuropsin and protease M/neurosin has different roles in the response of the spinal cord to injury as well as in normal homeostasis. (C) 2004 Wiley-Liss, Inc.