Colocalization of mineralocorticoid receptor and glucocorticoid receptor in the hippocampus and hypothalamus
Colocalization of mineralocorticoid receptor and glucocorticoid receptor in the hippocampus and hypothalamus
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DOI：
10.1016/j.neures.2004.12.013
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发表时间：
2005-04-01
影响因子：
2.9
通讯作者：
Kawata, M
中科院分区：
文献类型：
--
作者：
Han, F;Ozawa, H;Kawata, M
本研究采用双免疫荧光组织化学方法，结合特异性多克隆抗体，研究了皮质酮在大鼠脑内的主要靶区海马和下丘脑中的两种皮质类固醇受体，即矿化皮质激素受体（MR）和糖皮质激素受体（GR）的分布和共定位模式。MR-和gr -免疫反应性（ir）共定位于CA1和CA2锥体神经元和齿状回颗粒细胞，而MR-ir仅在CA3锥体神经元中可见。MR-和GR-ir的共定位也见于室旁核（PVN）的旁细胞区，但未见于室旁核的大细胞区。与GR-ir相比，MR-ir的亚细胞分布更倾向于细胞质分布，而这些受体的细胞质分布与核分布的比例在不同区域有所不同。肾上腺切除术（ADX）后，两种受体的分布模式均改变为细胞质分布，但各区域分布变化程度不同。ADX后皮质酮的替代恢复了假手术动物的分布模式。这些结果表明，细胞中MR和GR的平衡是通过海马和下丘脑对皮质类固醇进行潜在调节的基础。(c) 2004爱思唯尔爱尔兰有限公司和日本神经科学学会。版权所有。
We investigated the distribution and colocalization pattern of the two corticosteroid receptors, mineralocorticoid receptor (MR) and glucocorticoid receptor (GR), in the hippocampus and hypothalamus, the main target regions of corticosterone in the rat brain, using double immunofluorescence histochemistry in conjunction with specific polyclonal antibodies against MR and GR. In the hippocampus, MR- and GR-immunoreactivity (ir) were colocalized in CA1 and CA2 pyramidal neurons and granule cells of the dentate gyrus, while only MR-ir was seen in the CA3 pyramidal neurons. Colocalization of MR- and GR-ir was also observed in the parvocellular region, but not in the magnocellular region of the paraventricular nucleus (PVN). Subcellular distribution of MR-ir was more cytoplasmic in comparison with that of GR-ir, while the ratio of cytoplasmic to nuclear distribution of these receptors was different among the regions. After adrenalectomy (ADX), the distribution pattern of both receptors was changed to cytoplasmic, although the degree of the change of distribution was different among each region. Replacement of corticosterone after ADX recovered the distribution pattern to that of the sham-operated animals. These results suggest that the balance of MR and GR in the cell underlies the potential regulation of corticosteroid through the hippocampus and hypothalamus. (c) 2004 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.