Calcium-dependent regulatory protein of cyclic nucleotide metabolism in normal and transformed chicken embryo fibroblasts.
Calcium-dependent regulatory protein of cyclic nucleotide metabolism in normal and transformed chicken embryo fibroblasts.
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正常和转化鸡胚成纤维细胞中环核苷酸代谢的钙依赖性调节蛋白。
DOI：
10.1073/pnas.73.8.2711
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发表时间：
1976
影响因子：
11.1
通讯作者：
T. Vanaman
中科院分区：
文献类型：
--
作者：
D. M. Watterson;L. Eldik;E. Ralph;Smith;T. Vanaman
鸡胚成纤维细胞经劳斯肉瘤病毒转化后，3':5'-环核苷酸磷酸二酯酶（EC 3.1.4.17；3':5'-环核苷酸5'-核苷酸水解酶）活性的钙结合蛋白调节剂浓度增加。这种来自成纤维细胞的调节蛋白与从鸡脑中分离的调节蛋白具有大致相同的分子大小、电荷和功能特性，约占劳斯肉瘤病毒感染和转化的成纤维细胞匀浆中可溶性蛋白的 1.32%。相比之下，调节剂包含来自汇合培养物的正常成纤维细胞匀浆中约0.30%的可溶性蛋白，以及感染劳斯肉瘤病毒转化缺陷突变体的成纤维细胞匀浆中约0.36%的可溶性蛋白。正常成纤维细胞中亚汇合细胞密度下的调节剂水平为匀浆可溶性蛋白的0.42-0.76%，即介于汇合正常成纤维细胞和劳斯肉瘤病毒转化的成纤维细胞中发现的水平之间。这些观察结果表明，在鸡胚成纤维细胞快速生长且腺苷3':5'-环单磷酸水平降低的条件下，调节蛋白的水平升高。
The concentration of a calcium-binding protein modulator of 3':5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17; 3':5'-cyclic-nucleotide 5'-nucleotidohydrolase) activity is increased in chicken embryo fibroblasts upon transformation by Rous sarcoma virus. This modulator protein from fibroblasts, which has roughly the same molecular size, charge, and functional properties as that isolated from chicken brain, comprises approximately 1.32% of the soluble protein in homogenates of fibroblasts infected and transformed by Rous sarcoma virus. In comparison, the modulator comprises approximately 0.30% of the soluble protein in homogenates of normal fibroblasts from confluent cultures and 0.36% of the soluble protein in homogenates of fibroblasts infected with a transformation-defective mutant of Rous sarcoma virus. Modulator levels in normal fibroblasts at subconfluent cell densities are 0.42-0.76% of the homogenate soluble protein, i.e., between that found in confluent normal fibroblasts and in fibroblasts transformed by Rous sarcoma virus. These observations suggest that the levels of the modulator protein are elevated under conditions in which chicken embryo fibroblasts are undergoing rapid growth and have decreased adenosine 3':5'-cyclic monophosphate levels.