Excretion of trimethylselenonium ion in human urine.
Excretion of trimethylselenonium ion in human urine.
复制标题
三甲基硒离子在人尿中的排泄。
DOI：
10.1016/0003-2697(87)90169-2
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发表时间：
1987
影响因子：
2.9
通讯作者：
Janghorbani,M
中科院分区：
文献类型：
--
作者：
Sun,XF;Ting,BT;Janghorbani,M
建立了一种从1升人尿中分离和测定三甲基硒离子[TME，(CH3)3Se+]的方法。该方法是用硝酸铵沉淀，阴离子交换树脂预分离，最后在硝酸中热分解和收集产物。使用体内标记的大鼠尿液和添加了未标记的TM E的人尿液，测试了它的回收率和与尿液中存在的其他硒部分的分离。前者的回收率为76.8-87.0%(平均±SD：81.8±3.7%，n=5)，后者为72.0-93.0%(三次平均±SD分别为80.9±5.5、81.4±7.8和78.9±1.0)。使用体内标记的大鼠尿液对该方法的可靠性和高效液相色谱法进行了测试。目前尿中放射性的平均(±SD)百分率为36.0±5.7%，而高效液相色谱法为36.2±6.6%。相对标准偏差(−)为0.03±8.8%。两种方法的线性回归方程为y=−0.805+1.029x(R2=0.81)。测定了几个人的尿样(范围：0.18~0.37μg Se/L；平均±SD：0.2 6±0.0 7，n=9)。一名受试者在隔天(第一天，197μg Se；第三天，第395天；和第五天，592年)摄入了三种不同剂量的未标记亚硒酸盐。在每个周期的前24 h，TM E排泄量(μg Se/24 h)分别为0.24、0.53和0.97。24至48小时收集的相应数字分别为0.13、0.34和0.53μg/24小时。当以尿硒百分比表示时，范围为0.14-0.44%。结果表明，该方法能准确测定大容量(1升)人尿中的总硒含量，在典型的北美人群中，总硒只占尿硒的一小部分。
A method to permit isolation and measurement of trimethylselenonium ion [TMSe, (CH3)3Se+] from 1 liter of human urine was developed. The method was based on precipitation of TMSe with ammonium reineckate, preseparation with anion-exchange resin, and final thermal decomposition and collection of the product in HNO3. It was tested for recovery and separation from other selenium moieties present in urine using both in vivo-labeled rat urine and human urine spiked with unlabeled TMSe. Recoveries from the former were in the range 76.8–87.0% (mean ± SD: 81.8 ± 3.7%, n = 5), while for the latter they were in the range 72.0–93.0% (mean ± SD for three occasions (%): 80.9 ± 5.5, 81.4 ± 7.8, and 78.9 ± 1.0). The reliability of the method was tested against and HPLC procedure using in vivo-labeled rat's urine. The mean (±SD) percentage of urine radioactivity appearing as TMSe was 36.0 ± 5.7% for the present and 36.2 ± 6.6% for the HPLC method. The mean of deviations, as percentage of the HPLC method, was −0.03 ± 8.8%. The linear regression equation for the two methods was y = −0.805 + 1.029x (r2= 0.81). Excretion of TMSe was measured in urine samples from several persons (range: 0.18–0.37 μg Se/liter; mean ± SD: 0.26 ± 0.07, n = 9). One subject consumed three separate doses of unlabeled selenite on alternate days (Day 1, 197 μg Se; Day 3, 395; and Day 5, 592). For the first 24 h of each period, TMSe excretions (μg Se/24 h) were 0.24, 0.53, and 0.97, respectively. The corresponding figures for the 24- to 48-h collections were 0.13, 0.34, and 0.53 μg/24 h, respectively. When expressed as percentage of urine Se the range was 0.14–0.44%. It is concluded that this method is reliable for accurate measurement of TMSe from a large (1 liter) volume of human urine, and TMSe constitutes a quantitatively minor fraction of urine selenium in typical North Americans.