Studies in fat grafting: Part I. Effects of injection technique on in vitro fat viability and in vivo volume retention.
Studies in fat grafting: Part I. Effects of injection technique on in vitro fat viability and in vivo volume retention.
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DOI：
10.1097/prs.0000000000000290
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发表时间：
2014-07
影响因子：
3.6
通讯作者：
Longaker MT
中科院分区：
文献类型：
--
作者：
Chung MT;Paik KJ;Atashroo DA;Hyun JS;McArdle A;Senarath-Yapa K;Zielins ER;Tevlin R;Duldulao C;Hu MS;Walmsley GG;Parisi-Amon A;Momeni A;Rimsa JR;Commons GW;Gurtner GC;Wan DC;Longaker MT
脂肪移植在全身许多部位的软组织缺损矫正中越来越受欢迎。然而，长期结果取决于以最小创伤的方式输送脂肪，以优化移植组织的生存能力。在这项研究中，我们比较了两种方法注射后脂肪的生物学特性。从5名女性供体中获得抽脂样本，并使用改进的Coleman技术或自动低剪切装置评估注射后的细胞活力、增殖和脂肪分解。与最低限度加工、未注射的脂肪进行比较。使用改良的Coleman技术或脂肪组织注射器在免疫缺陷小鼠头皮下注射脂肪12周后，也测量了体积保留。最后，对脂肪移植进行组织学分析。脂肪组织注入器与改良的Coleman技术相比，脂肪活力和细胞增殖都显著增加。相比之下，使用自动化设备可以显著减少脂肪分解。在4、6、8和12周时间点，体内脂肪体积保留率显著高于改良Coleman技术。这与注射该装置后健康脂肪的组织学评分明显较高，损伤评分较低相对应。注射组织的生物学特性反映了脂肪植入技术的破坏性和有害程度，我们的体外和体内数据都支持与改进的Coleman技术相比使用自动化的低剪切装置。
Fat grafting has become increasingly popular for the correction of soft tissue deficits at many sites throughout the body. Long-term outcomes, however, depend on delivery of fat in the least traumatic fashion to optimize viability of the transplanted tissue. In this study, we compare the biologic properties of fat following injection using two methods. Lipoaspiration samples were obtained from five female donors and cellular viability, proliferation, and lipolysis were evaluated following injection using either a modified Coleman technique or an automated, low shear device. Comparisons were made to minimally processed, uninjected fat. Volume retention was also measured over twelve weeks following injection of fat under the scalp of immunodeficient mice using either the modified Coleman technique or the Adipose Tissue Injector. Finally, fat grafts were analyzed histologically. Fat viability and cellular proliferation were both significantly greater with the Adipose Tissue Injector relative to injection with the modified Coleman technique. In contrast, significantly less lipolysis was noted using the automated device. In vivo fat volume retention was significantly greater than with the modified Coleman technique at 4, 6, 8, and 12 week time points. This corresponded with significantly greater histological scores for healthy fat and lower scores for injury following injection with the device. Biological properties of injected tissues reflect how disruptive and harmful techniques for placement of fat may be, and our in vitro and in vivo data both support the use of the automated, low shear devices compared to the modified Coleman technique.